Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 148183..148919 Replicon plasmid pKp199-1
Accession NZ_CP035536
Organism Klebsiella pneumoniae subsp. pneumoniae strain CCRI-22199

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag Kp711_RS00865 Protein ID WP_003026803.1
Coordinates 148183..148665 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag Kp711_RS00870 Protein ID WP_003026799.1
Coordinates 148653..148919 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Kp711_RS00845 143537..145300 - 1764 WP_004200842.1 DUF262 domain-containing protein -
Kp711_RS00850 145498..145704 - 207 Protein_138 helix-turn-helix domain-containing protein -
Kp711_RS00855 145918..146550 + 633 WP_001567369.1 hypothetical protein -
Kp711_RS00860 146579..147982 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
Kp711_RS00865 148183..148665 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
Kp711_RS00870 148653..148919 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
Kp711_RS00875 149164..149598 - 435 WP_001567367.1 cell envelope integrity protein TolA -
Kp711_RS00880 149645..150193 - 549 WP_001567366.1 thioredoxin fold domain-containing protein -
Kp711_RS00890 150687..150995 + 309 WP_017896554.1 hypothetical protein -
Kp711_RS00900 151894..153432 - 1539 WP_004201219.1 IS66-like element ISKpn24 family transposase -
Kp711_RS00905 153481..153828 - 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / mph(A) / tet(A) - 1..185957 185957
- inside IScluster/Tn - - 146579..154229 7650


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T118088 WP_003026803.1 NZ_CP035536:c148665-148183 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T118088 NZ_CP035536:c148665-148183 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT118088 WP_003026799.1 NZ_CP035536:c148919-148653 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT118088 NZ_CP035536:c148919-148653 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATTCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References