Detailed information of TA system TA118084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 377450..378087
• Replicon: chromosome
• Accession: NZ_CP035533
• Organism: Bacillus velezensis strain DTU001

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: EU243_RS01920
• Protein ID: WP_003156187.1
• Coordinates: 377450..377800 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: EU243_RS01925
• Protein ID: WP_003156188.1
• Coordinates: 377806..378087 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EU243_RS01880	372490..373092	-	603	WP_003156170.1	SpoIIE family protein phosphatase	-
EU243_RS01885	373092..373880	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
EU243_RS01890	373846..374328	-	483	WP_003156173.1	anti-sigma B factor RsbW	-
EU243_RS01895	374325..374654	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
EU243_RS01900	374718..375725	-	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
EU243_RS01905	375737..376138	-	402	WP_003156178.1	anti-sigma regulatory factor	-
EU243_RS01910	376141..376506	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
EU243_RS01915	376511..377332	-	822	WP_003156182.1	STAS domain-containing protein	-
EU243_RS01920	377450..377800	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
EU243_RS01925	377806..378087	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
EU243_RS01930	378207..379376	-	1170	WP_015388675.1	alanine racemase	-
EU243_RS01935	379493..380500	-	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
EU243_RS01940	380665..381030	-	366	WP_048367687.1	holo-ACP synthase	-
EU243_RS01945	381123..381722	+	600	WP_003156193.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T118084 WP_003156187.1 NZ_CP035533:c377800-377450 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T118084 NZ_CP035533:c377800-377450 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT118084 WP_003156188.1 NZ_CP035533:c378087-377806 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT118084 NZ_CP035533:c378087-377806 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5