Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4527300..4527925 Replicon chromosome
Accession NZ_CP035531
Organism Klebsiella pneumoniae strain NFYY0065

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag EI683_RS22540 Protein ID WP_002882817.1
Coordinates 4527542..4527925 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag EI683_RS22535 Protein ID WP_004150355.1
Coordinates 4527300..4527542 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EI683_RS22510 4523291..4523890 + 600 WP_004151865.1 glucose-1-phosphatase -
EI683_RS22515 4523884..4524744 + 861 WP_004146232.1 virulence factor BrkB family protein -
EI683_RS22520 4524741..4525178 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
EI683_RS22525 4525223..4526164 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
EI683_RS22530 4526178..4527095 - 918 WP_023302328.1 alpha/beta hydrolase -
EI683_RS22535 4527300..4527542 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
EI683_RS22540 4527542..4527925 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
EI683_RS22545 4528099..4529028 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
EI683_RS22550 4529025..4529660 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
EI683_RS22555 4529657..4530559 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T118080 WP_002882817.1 NZ_CP035531:4527542-4527925 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T118080 NZ_CP035531:4527542-4527925 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT118080 WP_004150355.1 NZ_CP035531:4527300-4527542 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT118080 NZ_CP035531:4527300-4527542 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References