Detailed information of TA system TA118051

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4939214..4940034
• Replicon: chromosome
• Accession: NZ_CP035516
• Organism: Escherichia coli strain U14A

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: U14A_RS24600
• Protein ID: WP_001054379.1
• Coordinates: 4939214..4939471 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: U14A_RS24605
• Protein ID: WP_000123957.1
• Coordinates: 4939483..4940034 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U14A_RS24585	4937076..4937330	-	255	WP_000788354.1	glycosyltransferase	-
U14A_RS24590	4937493..4938640	+	1148	WP_085949154.1	IS3-like element ISEc52 family transposase	-
U14A_RS24595	4938673..4938837	+	165	Protein_4701	GNAT family N-acetyltransferase	-
U14A_RS24600	4939214..4939471	+	258	WP_001054379.1	YjhX family toxin	Toxin
U14A_RS24605	4939483..4940034	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
U14A_RS24610	4940086..4940832	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
U14A_RS24615	4940960..4941220	+	261	WP_000077645.1	hypothetical protein	-
U14A_RS27155	4941258..4941374	+	117	Protein_4706	VOC family protein	-
U14A_RS24620	4941619..4942740	+	1122	WP_000010833.1	M42 family metallopeptidase	-
U14A_RS24625	4942737..4943015	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
U14A_RS24630	4943027..4944340	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4926984..4948255	21271

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T118051 WP_001054379.1 NZ_CP035516:4939214-4939471 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T118051 NZ_CP035516:4939214-4939471 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT118051 WP_000123957.1 NZ_CP035516:4939483-4940034 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT118051 NZ_CP035516:4939483-4940034 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030