Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4922320..4923155 Replicon chromosome
Accession NZ_CP035516
Organism Escherichia coli strain U14A

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag U14A_RS24515 Protein ID WP_000854759.1
Coordinates 4922778..4923155 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag U14A_RS24510 Protein ID WP_001295723.1
Coordinates 4922320..4922688 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U14A_RS24475 4917448..4918194 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
U14A_RS24480 4918209..4919750 - 1542 WP_001298859.1 IS21-like element ISEc12 family transposase -
U14A_RS24485 4919946..4920566 + 621 Protein_4680 DUF932 domain-containing protein -
U14A_RS24495 4920908..4921381 + 474 WP_001350782.1 antirestriction protein -
U14A_RS24500 4921397..4921873 + 477 WP_001186775.1 RadC family protein -
U14A_RS24505 4921936..4922157 + 222 WP_000692345.1 DUF987 domain-containing protein -
U14A_RS24510 4922320..4922688 + 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
U14A_RS24515 4922778..4923155 + 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
U14A_RS27000 4923152..4923322 + 171 WP_158604577.1 hypothetical protein -
U14A_RS24520 4923337..4923762 + 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
U14A_RS24525 4923759..4924109 + 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
U14A_RS24530 4924140..4925742 + 1603 Protein_4689 IS66-like element ISEc23 family transposase -
U14A_RS24535 4925774..4926169 + 396 Protein_4690 hypothetical protein -
U14A_RS24540 4926186..4926362 + 177 WP_000839286.1 DUF957 domain-containing protein -
U14A_RS27005 4926462..4926620 + 159 WP_001467148.1 hypothetical protein -
U14A_RS24550 4926984..4927160 + 177 Protein_4693 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4926984..4948255 21271
- inside IScluster/Tn - - 4917448..4925742 8294


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T118050 WP_000854759.1 NZ_CP035516:4922778-4923155 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T118050 NZ_CP035516:4922778-4923155 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT118050 WP_001295723.1 NZ_CP035516:4922320-4922688 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT118050 NZ_CP035516:4922320-4922688 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References