Detailed information of TA system TA118021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-Phd
• Location: 133435..134120
• Replicon: plasmid pOT1-5
• Accession: NZ_CP035515
• Organism: Haematobacter massiliensis strain OT1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HmaOT1_RS19810
• Protein ID: WP_131390049.1
• Coordinates: 133435..133857 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4P6S2P8
• Locus tag: HmaOT1_RS19815
• Protein ID: WP_045022299.1
• Coordinates: 133854..134120 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HmaOT1_RS19770	128588..129145	-	558	WP_036568189.1	phosphonate C-P lyase system protein PhnH	-
HmaOT1_RS19775	129145..129600	-	456	WP_107328120.1	phosphonate C-P lyase system protein PhnG	-
HmaOT1_RS19780	129597..130343	-	747	WP_024899717.1	phosphonate metabolism transcriptional regulator PhnF	-
HmaOT1_RS19785	130699..131844	-	1146	WP_131390111.1	site-specific integrase	-
HmaOT1_RS19790	131834..132253	-	420	WP_131390045.1	type II toxin-antitoxin system VapC family toxin	-
HmaOT1_RS19795	132253..132492	-	240	WP_131390112.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
HmaOT1_RS20285	132753..133397	-	645	WP_155045811.1	hypothetical protein	-
HmaOT1_RS19810	133435..133857	-	423	WP_131390049.1	type II toxin-antitoxin system VapC family toxin	Toxin
HmaOT1_RS19815	133854..134120	-	267	WP_045022299.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HmaOT1_RS19820	134209..135342	-	1134	WP_131390114.1	site-specific integrase	-
HmaOT1_RS19825	135468..136421	+	954	WP_003501255.1	DUF1403 family protein	-
HmaOT1_RS19830	136424..137134	+	711	WP_107327914.1	SMC-Scp complex subunit ScpB	-
HmaOT1_RS19835	137144..137776	-	633	WP_035243799.1	hypothetical protein	-
HmaOT1_RS19840	137806..138867	+	1062	Protein_134	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..183422	183422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-71)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15577.98 Da
• Isoelectric Point: 5.6239

>T118021 WP_131390049.1 NZ_CP035515:c133857-133435 [Haematobacter massiliensis]
MRLLLDTNVLSEVTKPRPDEAVLKWLHGLDEDRTFISIVSIAEIRRGVALMDAGRKRDALNEWLAHDLPQRFESRIIPVE
GHVALAWGDLMALAKRSGRGLASMDGLIAATAIAHQLTLATRNIKDFEGFGIDIIDPWVD

Nucleotide

• Length: 423 bp

>T118021 NZ_CP035515:c133857-133435 [Haematobacter massiliensis]
ATGAGGCTGCTCCTCGATACGAACGTTCTGTCGGAAGTTACGAAACCTCGCCCGGATGAAGCGGTTCTTAAGTGGCTTCA
CGGGCTGGACGAAGACCGTACGTTCATCAGCATCGTGTCGATCGCTGAAATTCGCCGCGGTGTCGCGCTGATGGATGCTG
GGCGAAAACGCGATGCCCTGAACGAATGGCTCGCGCATGATCTGCCTCAACGCTTCGAAAGCAGGATTATTCCGGTGGAA
GGGCATGTCGCCTTGGCCTGGGGCGATCTCATGGCACTTGCCAAGCGAAGTGGTCGGGGGCTTGCCTCGATGGATGGTCT
GATTGCCGCCACAGCCATCGCTCACCAGTTGACCCTTGCGACGCGCAACATCAAGGATTTCGAGGGCTTTGGAATAGACA
TCATTGACCCATGGGTGGACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9757.01 Da
• Isoelectric Point: 6.4684

>AT118021 WP_045022299.1 NZ_CP035515:c134120-133854 [Haematobacter massiliensis]
MQASTHEDMSWTVAGAKAKLSEVMERAQLAPQTITRNGKPSVVVVSAEEWQRKTGRRGSLAEFLLASPLRGADLDLERQH
DEPRDLSL

Nucleotide

• Length: 267 bp

>AT118021 NZ_CP035515:c134120-133854 [Haematobacter massiliensis]
ATGCAAGCATCAACGCACGAAGATATGAGCTGGACGGTCGCTGGCGCGAAGGCAAAGCTCTCGGAAGTTATGGAACGTGC
GCAATTGGCGCCTCAGACAATTACCCGCAACGGAAAGCCGAGCGTGGTCGTCGTTTCCGCAGAAGAGTGGCAGCGGAAAA
CTGGGCGTAGGGGATCGCTCGCGGAATTTCTGTTGGCATCGCCTCTGCGCGGCGCGGATCTGGATCTTGAACGGCAGCAC
GACGAGCCACGTGACTTGTCGTTATGA

Structures

Antitoxin

• AlphaFold DB: A0A4P6S2P8