Detailed information of TA system TA118019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 17044..17699
• Replicon: plasmid pOT1-9
• Accession: NZ_CP035509
• Organism: Haematobacter massiliensis strain OT1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A086XSA4
• Locus tag: HmaOT1_RS01485
• Protein ID: WP_035715247.1
• Coordinates: 17044..17463 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HmaOT1_RS01490
• Protein ID: WP_084684284.1
• Coordinates: 17460..17699 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HmaOT1_RS01465	13297..14229	-	933	WP_076534770.1	IS5-like element ISPko1 family transposase	-
HmaOT1_RS01470	14546..15406	-	861	Protein_14	IS110 family transposase	-
HmaOT1_RS01475	15472..16607	+	1136	WP_131389364.1	IS3 family transposase	-
HmaOT1_RS01480	16669..16839	-	171	Protein_16	transposase	-
HmaOT1_RS01485	17044..17463	-	420	WP_035715247.1	type II toxin-antitoxin system VapC family toxin	Toxin
HmaOT1_RS01490	17460..17699	-	240	WP_084684284.1	hypothetical protein	Antitoxin
HmaOT1_RS01495	18008..18310	-	303	WP_131389366.1	hypothetical protein	-
HmaOT1_RS01500	18798..20135	+	1338	WP_035715267.1	DUF3560 domain-containing protein	-
HmaOT1_RS01505	20132..20734	+	603	WP_035715265.1	methyltransferase type 11	-
HmaOT1_RS20055	21356..21514	+	159	WP_156969595.1	hypothetical protein	-
HmaOT1_RS01510	21549..21983	-	435	WP_165496666.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..54639	54639

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15271.63 Da
• Isoelectric Point: 4.8311

>T118019 WP_035715247.1 NZ_CP035509:c17463-17044 [Haematobacter massiliensis]
MIILDTNVISETQKPKPDPKVMAWLDTLDMSSTYLTAITVAELFFGVDQLPEGKRKTVLSDGVSHLIETIFYGRILPFDA
TAAYFFGSMMADARKQGHTITFPDGAIAAIARGNNFAAVATRDVTPFAAMKIEIINPWE

Nucleotide

• Length: 420 bp

>T118019 NZ_CP035509:c17463-17044 [Haematobacter massiliensis]
ATGATTATCCTCGATACGAATGTGATTTCCGAAACGCAGAAGCCGAAACCGGATCCGAAGGTCATGGCATGGCTCGACAC
GCTGGACATGTCGTCCACCTATCTGACTGCGATCACGGTGGCAGAGCTATTCTTTGGGGTCGATCAACTTCCCGAGGGCA
AGCGAAAGACTGTTTTGAGCGATGGGGTAAGCCATCTGATAGAAACGATCTTTTACGGTCGCATCCTTCCCTTCGATGCC
ACGGCTGCATATTTTTTTGGGTCCATGATGGCGGACGCCCGCAAACAGGGGCATACGATCACCTTTCCTGACGGTGCTAT
CGCGGCAATCGCACGAGGGAACAACTTCGCTGCGGTCGCCACCCGCGATGTGACGCCCTTCGCGGCTATGAAGATAGAGA
TTATCAATCCGTGGGAGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8642.54 Da
• Isoelectric Point: 6.0611

>AT118019 WP_084684284.1 NZ_CP035509:c17699-17460 [Haematobacter massiliensis]
MGNVTIRNVPDEVHTALKLRAVRHQRSTEAEMRAILTAAVSQETGNGLGQQMRSAWSGHHGSDFDDLRDPSPVEGATFE

Nucleotide

• Length: 240 bp

>AT118019 NZ_CP035509:c17699-17460 [Haematobacter massiliensis]
ATGGGAAATGTCACCATTCGCAACGTTCCAGATGAAGTTCACACTGCCCTGAAGCTCCGTGCCGTTCGTCATCAGCGCAG
CACGGAAGCAGAGATGCGAGCTATTCTGACGGCCGCCGTTTCCCAAGAAACAGGGAATGGGCTTGGTCAGCAGATGCGGT
CCGCATGGTCAGGACATCACGGCAGCGATTTTGACGATCTGCGCGACCCGTCACCCGTGGAAGGCGCAACTTTCGAATGA

Structures

Toxin

• AlphaFold DB: A0A086XSA4