Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3771329..3771915 Replicon chromosome
Accession NZ_CP035503
Organism Rhodoferax sediminis strain CHu59-6-5

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag EUB48_RS18210 Protein ID WP_142820442.1
Coordinates 3771571..3771915 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag EUB48_RS18205 Protein ID WP_210411661.1
Coordinates 3771329..3771571 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EUB48_RS18175 3766504..3767583 + 1080 WP_142820436.1 DUF3108 domain-containing protein -
EUB48_RS18180 3767612..3768910 - 1299 WP_142820437.1 phenylacetate--CoA ligase -
EUB48_RS18185 3768907..3769344 - 438 WP_142820438.1 hydroxyphenylacetyl-CoA thioesterase PaaI -
EUB48_RS18190 3769319..3770146 - 828 WP_142820439.1 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase PaaG -
EUB48_RS18195 3770262..3771041 + 780 WP_142820440.1 enoyl-CoA hydratase -
EUB48_RS18205 3771329..3771571 + 243 WP_210411661.1 PbsX family transcriptional regulator Antitoxin
EUB48_RS18210 3771571..3771915 + 345 WP_142820442.1 endoribonuclease MazF Toxin
EUB48_RS18215 3772114..3772479 + 366 WP_142820443.1 hypothetical protein -
EUB48_RS18220 3772587..3773930 + 1344 WP_142820444.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
EUB48_RS18225 3774006..3775370 - 1365 WP_142820445.1 signal recognition particle protein -
EUB48_RS18230 3775467..3776276 + 810 WP_142820446.1 cytochrome c biogenesis protein CcsA -
EUB48_RS18235 3776288..3776503 + 216 WP_142821399.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(1-80)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12445.63 Da        Isoelectric Point: 10.3642

>T118015 WP_142820442.1 NZ_CP035503:3771571-3771915 [Rhodoferax sediminis]
MATRGYVPDSGDVVWLQFDPRAGHEQAGHRPALVISPAAYNAKSGLMVCCPMSTKIKGHPFEVVTEVDGVPSAILSDQVK
SLDWKVRRAKRKSAVSPEVMLHVRAKIKALLVLK

Download         Length: 345 bp

>T118015 NZ_CP035503:3771571-3771915 [Rhodoferax sediminis]
ATGGCGACTCGCGGGTATGTCCCGGACAGTGGCGACGTGGTCTGGCTGCAATTTGATCCGCGAGCCGGCCACGAGCAGGC
GGGACACCGCCCGGCGCTGGTGATCAGCCCGGCGGCCTACAACGCCAAGTCCGGTCTGATGGTCTGCTGCCCGATGTCCA
CGAAGATCAAGGGGCACCCTTTTGAAGTCGTAACCGAGGTGGATGGCGTTCCAAGCGCCATCCTGTCCGATCAGGTGAAG
TCCCTTGACTGGAAGGTTCGTCGAGCCAAGCGGAAATCCGCCGTGAGCCCTGAGGTCATGCTTCACGTCCGGGCCAAGAT
CAAAGCACTTCTTGTGCTCAAATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8721.22 Da        Isoelectric Point: 7.2029

>AT118015 WP_210411661.1 NZ_CP035503:3771329-3771571 [Rhodoferax sediminis]
MEAVIKKWGNSPAVRLPVALMKLATLTLEQKVNVTAVEGKIIIEPLDHVEYRLEDLVKGITRSNSHAEVSFGAPVGKEAL

Download         Length: 243 bp

>AT118015 NZ_CP035503:3771329-3771571 [Rhodoferax sediminis]
ATGGAAGCCGTCATCAAAAAATGGGGAAACAGCCCCGCCGTTCGCCTCCCCGTCGCCCTCATGAAGCTCGCCACGCTCAC
CCTTGAGCAAAAGGTGAATGTCACGGCCGTGGAAGGCAAGATCATCATCGAGCCTCTGGACCATGTCGAGTACCGCCTCG
AGGATCTTGTCAAAGGCATCACGCGCAGCAACTCGCATGCCGAAGTCAGCTTCGGCGCTCCGGTTGGCAAGGAAGCCCTC
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References