Detailed information of TA system TA118008

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4826019..4826851
• Replicon: chromosome
• Accession: NZ_CP035498
• Organism: Escherichia coli strain 04-00955

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2R9V8K2
• Locus tag: EQO00_RS24005
• Protein ID: WP_001468432.1
• Coordinates: 4826477..4826851 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: EQO00_RS24000
• Protein ID: WP_001285575.1
• Coordinates: 4826019..4826387 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQO00_RS23965	4821912..4822592	+	681	WP_001282919.1	WYL domain-containing protein	-
EQO00_RS23970	4822740..4823417	+	678	WP_001097301.1	hypothetical protein	-
EQO00_RS23975	4823423..4823656	+	234	WP_001278283.1	DUF905 family protein	-
EQO00_RS23980	4823746..4824564	+	819	WP_001175160.1	DUF945 domain-containing protein	-
EQO00_RS23985	4824655..4825140	+	486	WP_000213697.1	antirestriction protein	-
EQO00_RS23990	4825155..4825631	+	477	WP_001298074.1	RadC family protein	-
EQO00_RS23995	4825718..4825939	+	222	WP_000692350.1	DUF987 domain-containing protein	-
EQO00_RS24000	4826019..4826387	+	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EQO00_RS24005	4826477..4826851	+	375	WP_001468432.1	TA system toxin CbtA family protein	Toxin
EQO00_RS24010	4826848..4827336	+	489	WP_000777545.1	hypothetical protein	-
EQO00_RS24015	4827348..4827545	+	198	WP_000839281.1	DUF957 domain-containing protein	-
EQO00_RS24020	4827642..4828211	+	570	WP_001290252.1	DUF4942 domain-containing protein	-
EQO00_RS24030	4828960..4830498	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4807592..4838311	30719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14102.04 Da
• Isoelectric Point: 7.3526

>T118008 WP_001468432.1 NZ_CP035498:4826477-4826851 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T118008 NZ_CP035498:4826477-4826851 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT118008 WP_001285575.1 NZ_CP035498:4826019-4826387 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT118008 NZ_CP035498:4826019-4826387 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2R9V8K2

Antitoxin

• AlphaFold DB: A0A454ABF5