Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4361276..4361826 Replicon chromosome
Accession NZ_CP035498
Organism Escherichia coli strain 04-00955

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag EQO00_RS21645 Protein ID WP_001160966.1
Coordinates 4361276..4361590 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag EQO00_RS21650 Protein ID WP_000125566.1
Coordinates 4361593..4361826 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQO00_RS21620 4356951..4358237 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
EQO00_RS21625 4358237..4359226 + 990 WP_000241228.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
EQO00_RS21630 4359223..4360044 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
EQO00_RS21635 4360047..4360424 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
EQO00_RS21640 4360431..4361279 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
EQO00_RS21645 4361276..4361590 - 315 WP_001160966.1 CcdB family protein Toxin
EQO00_RS21650 4361593..4361826 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
EQO00_RS21655 4361912..4362391 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
EQO00_RS21660 4362583..4364445 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
EQO00_RS21665 4364438..4364968 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
EQO00_RS21670 4365076..4366407 - 1332 WP_001183207.1 MFS transporter -
EQO00_RS21675 4366466..4366753 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T118004 WP_001160966.1 NZ_CP035498:c4361590-4361276 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T118004 NZ_CP035498:c4361590-4361276 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT118004 WP_000125566.1 NZ_CP035498:c4361826-4361593 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT118004 NZ_CP035498:c4361826-4361593 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References