Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1053612..1054266 Replicon chromosome
Accession NZ_CP035498
Organism Escherichia coli strain 04-00955

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag EQO00_RS05165 Protein ID WP_000244781.1
Coordinates 1053859..1054266 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EQO00_RS05160 Protein ID WP_000354046.1
Coordinates 1053612..1053878 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQO00_RS05140 1049700..1051133 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
EQO00_RS05145 1051178..1051489 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
EQO00_RS05150 1051653..1052312 + 660 WP_000250274.1 hemolysin III family protein -
EQO00_RS05155 1052389..1053369 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
EQO00_RS05160 1053612..1053878 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EQO00_RS05165 1053859..1054266 + 408 WP_000244781.1 protein YgfX Toxin
EQO00_RS05170 1054306..1054827 - 522 WP_001055874.1 flavodoxin FldB -
EQO00_RS05175 1054939..1055835 + 897 WP_000806633.1 site-specific tyrosine recombinase XerD -
EQO00_RS05180 1055860..1056570 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EQO00_RS05185 1056576..1058309 + 1734 WP_000813193.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T117986 WP_000244781.1 NZ_CP035498:1053859-1054266 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T117986 NZ_CP035498:1053859-1054266 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT117986 WP_000354046.1 NZ_CP035498:1053612-1053878 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT117986 NZ_CP035498:1053612-1053878 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References