Detailed information of TA system TA117985

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 832383..833215
• Replicon: chromosome
• Accession: NZ_CP035498
• Organism: Escherichia coli strain 04-00955

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: EQO00_RS04070
• Protein ID: WP_000854753.1
• Coordinates: 832383..832757 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: EQO00_RS04075
• Protein ID: WP_001278232.1
• Coordinates: 832847..833215 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQO00_RS04040	827496..828644	-	1149	WP_000905931.1	capsule polysaccharide export inner-membrane protein KpsE	-
EQO00_RS04045	828716..829699	-	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-
EQO00_RS04050	830510..830674	-	165	Protein_791	IS110 family transposase	-
EQO00_RS04055	831023..831592	-	570	WP_096888569.1	DUF4942 domain-containing protein	-
EQO00_RS04060	831689..831886	-	198	WP_000839281.1	DUF957 domain-containing protein	-
EQO00_RS04065	831898..832386	-	489	WP_000777541.1	hypothetical protein	-
EQO00_RS04070	832383..832757	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
EQO00_RS04075	832847..833215	-	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
EQO00_RS04080	833378..833599	-	222	WP_000692316.1	DUF987 domain-containing protein	-
EQO00_RS04085	833662..834138	-	477	WP_001186726.1	RadC family protein	-
EQO00_RS04090	834154..834639	-	486	WP_044706283.1	antirestriction protein	-
EQO00_RS04095	834694..835512	-	819	WP_001234620.1	DUF945 domain-containing protein	-
EQO00_RS04105	835612..835845	-	234	WP_001119729.1	DUF905 family protein	-
EQO00_RS04110	835924..836379	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T117985 WP_000854753.1 NZ_CP035498:c832757-832383 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T117985 NZ_CP035498:c832757-832383 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT117985 WP_001278232.1 NZ_CP035498:c833215-832847 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT117985 NZ_CP035498:c833215-832847 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: E3PJ72