Detailed information of TA system TA117981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3625067..3625725
• Replicon: chromosome
• Accession: NZ_CP035494
• Organism: Microbacterium protaetiae strain DFW100M-13

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ET475_RS16935
• Protein ID: WP_129392998.1
• Coordinates: 3625067..3625471 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: ET475_RS16940
• Protein ID: WP_129393002.1
• Coordinates: 3625468..3625725 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ET475_RS16920	3621164..3621463	-	300	WP_129392989.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
ET475_RS16925	3621561..3623267	+	1707	WP_129392992.1	AMP-binding protein	-
ET475_RS16930	3623264..3625051	+	1788	WP_129392995.1	DEAD/DEAH box helicase	-
ET475_RS16935	3625067..3625471	-	405	WP_129392998.1	PIN domain-containing protein	Toxin
ET475_RS16940	3625468..3625725	-	258	WP_129393002.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ET475_RS16945	3625813..3628116	-	2304	WP_129393005.1	NAD-dependent DNA ligase LigA	-
ET475_RS16950	3628126..3629241	-	1116	WP_129393008.1	tRNA 2-thiouridine(34) synthase MnmA	-
ET475_RS16955	3629252..3630439	-	1188	WP_129393011.1	cysteine desulfurase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-41)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14783.85 Da
• Isoelectric Point: 4.4508

>T117981 WP_129392998.1 NZ_CP035494:c3625471-3625067 [Microbacterium protaetiae]
MSDESIGVVDTSIMIGFESGREIDFEKIPVGMVSTVITQAELEAGVLSTDDSQARSRRLETLEKLSEMRVLAVTREAAHH
WARLRLFLRDTGRNMQVNDLWIAAIAVANELPIVTQDADFDALEGAKGVTVIRV

Nucleotide

• Length: 405 bp

>T117981 NZ_CP035494:c3625471-3625067 [Microbacterium protaetiae]
ATGAGCGACGAATCCATCGGAGTCGTTGACACCTCGATCATGATCGGCTTCGAATCGGGCCGCGAGATCGACTTTGAGAA
GATCCCCGTCGGCATGGTCTCGACTGTGATCACACAGGCTGAGCTCGAAGCCGGGGTTCTCTCGACGGACGACTCGCAAG
CTCGATCGCGCCGCCTCGAGACCCTTGAGAAACTCAGTGAGATGCGCGTTCTCGCCGTGACGAGGGAAGCGGCTCATCAT
TGGGCACGACTGCGACTGTTCCTCCGGGATACGGGGCGGAACATGCAGGTCAACGACCTGTGGATCGCCGCGATCGCCGT
CGCGAACGAGCTGCCGATCGTGACGCAGGATGCCGACTTCGACGCGCTGGAAGGCGCGAAGGGCGTCACCGTCATCCGCG
TCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9530.90 Da
• Isoelectric Point: 4.8782

>AT117981 WP_129393002.1 NZ_CP035494:c3625725-3625468 [Microbacterium protaetiae]
MAEVPVRELRNDTAGVLRRAQAGEDIVITVHGNPAVRLIPAAPERRRVIPREELFAWLLKHQADPGLRDELRELAGEMTD
EADIV

Nucleotide

• Length: 258 bp

>AT117981 NZ_CP035494:c3625725-3625468 [Microbacterium protaetiae]
ATGGCTGAGGTACCTGTCCGAGAACTGCGCAACGACACCGCCGGCGTGCTCAGGCGCGCTCAGGCGGGGGAAGACATCGT
GATCACGGTGCATGGAAACCCCGCGGTCAGGCTCATTCCGGCCGCCCCCGAGCGGCGGCGCGTCATACCGCGTGAAGAGC
TCTTCGCCTGGCTGCTCAAACATCAAGCAGATCCTGGTCTTCGCGACGAACTCCGAGAGCTGGCGGGTGAGATGACCGAC
GAGGCGGACATCGTATGA