Detailed information of TA system TA117978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-StbC
• Location: 1739577..1740205
• Replicon: chromosome
• Accession: NZ_CP035494
• Organism: Microbacterium protaetiae strain DFW100M-13

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ET475_RS08050
• Protein ID: WP_129388356.1
• Coordinates: 1739577..1739981 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: ET475_RS08055
• Protein ID: WP_129388359.1
• Coordinates: 1739978..1740205 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ET475_RS08025	1735173..1736432	-	1260	WP_129388341.1	aminotransferase class V-fold PLP-dependent enzyme	-
ET475_RS08030	1736422..1737393	-	972	WP_129388344.1	hypothetical protein	-
ET475_RS08035	1737395..1738255	-	861	WP_129388347.1	tryptophan 2,3-dioxygenase	-
ET475_RS08040	1738385..1738708	-	324	WP_129388350.1	HEPN domain-containing protein	-
ET475_RS08045	1738853..1739446	-	594	WP_129388353.1	winged helix-turn-helix domain-containing protein	-
ET475_RS08050	1739577..1739981	-	405	WP_129388356.1	type II toxin-antitoxin system VapC family toxin	Toxin
ET475_RS08055	1739978..1740205	-	228	WP_129388359.1	toxin-antitoxin system, antitoxin component	Antitoxin
ET475_RS08060	1740396..1741904	-	1509	WP_129388362.1	HNH endonuclease	-
ET475_RS08065	1742421..1742675	-	255	WP_129388365.1	NrdH-redoxin	-
ET475_RS08070	1742672..1743388	-	717	WP_207205440.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
ET475_RS08075	1743454..1744398	+	945	WP_129388370.1	zinc-binding alcohol dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 13917.06 Da
• Isoelectric Point: 5.5424

>T117978 WP_129388356.1 NZ_CP035494:c1739981-1739577 [Microbacterium protaetiae]
MIVLDASAAVEWLLGRSGARFVAERIADPDVSIHAPAPIGVEVVSALRGLTLRHHMSADRGAAAIADLAAAGVDLHDPTP
LLPRAWQLRANLSAYDAVYVALAEVLDATLLTTDARIARAPGIAAEVVVVPTGK

Nucleotide

• Length: 405 bp

>T117978 NZ_CP035494:c1739981-1739577 [Microbacterium protaetiae]
ATGATCGTGCTGGATGCCTCGGCAGCAGTCGAGTGGCTGTTGGGCCGGAGTGGCGCCCGGTTCGTCGCCGAACGCATCGC
GGATCCAGATGTCTCGATCCATGCGCCCGCCCCGATCGGTGTCGAGGTGGTGTCTGCCCTGCGCGGACTCACGCTCAGGC
ACCACATGAGCGCAGATCGTGGTGCCGCCGCGATCGCCGACCTGGCTGCCGCCGGCGTCGACCTGCACGACCCCACCCCA
TTGCTTCCCCGCGCCTGGCAACTGCGCGCCAATCTGTCGGCGTACGACGCCGTGTACGTGGCACTCGCCGAGGTACTGGA
TGCCACTCTTCTCACGACGGACGCCCGCATCGCGCGGGCACCAGGCATCGCGGCCGAGGTCGTGGTCGTGCCGACGGGCA
AGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8286.50 Da
• Isoelectric Point: 9.1275

>AT117978 WP_129388359.1 NZ_CP035494:c1740205-1739978 [Microbacterium protaetiae]
MTMIQIRNVPDEVHRRLKARAAMEGVSLSELALAELRRSLDRPTRAELIDLVASRESVSDGPSSVELVTAARSTR

Nucleotide

• Length: 228 bp

>AT117978 NZ_CP035494:c1740205-1739978 [Microbacterium protaetiae]
ATGACAATGATCCAGATTCGCAACGTGCCCGATGAGGTGCACCGACGCCTCAAGGCGCGCGCAGCGATGGAGGGCGTGTC
ACTGTCAGAGCTCGCTTTGGCCGAGCTTCGGCGCTCCCTCGATCGGCCCACGCGCGCAGAGTTGATCGATCTCGTCGCGT
CTCGCGAGTCCGTCTCCGATGGTCCTTCTTCGGTCGAACTGGTGACCGCGGCGCGCTCGACACGATGA