Detailed information of TA system TA117973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-RHH
• Location: 2154856..2155448
• Replicon: chromosome
• Accession: NZ_CP035491
• Organism: Agromyces protaetiae strain FW100M-8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ET445_RS10015
• Protein ID: WP_129191077.1
• Coordinates: 2155077..2155448 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ET445_RS10010
• Protein ID: WP_129191075.1
• Coordinates: 2154856..2155080 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ET445_RS09995	2150647..2151702	+	1056	WP_129191069.1	glycosyltransferase family 2 protein	-
ET445_RS10000	2151708..2153420	+	1713	WP_129191071.1	glycosyltransferase	-
ET445_RS10005	2153514..2154815	+	1302	WP_129191073.1	CDP-glycerol glycerophosphotransferase family protein	-
ET445_RS10010	2154856..2155080	+	225	WP_129191075.1	ribbon-helix-helix domain-containing protein	Antitoxin
ET445_RS10015	2155077..2155448	+	372	WP_129191077.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ET445_RS10020	2155462..2158626	-	3165	WP_129191079.1	type I restriction endonuclease subunit R	-
ET445_RS10025	2158626..2159927	-	1302	WP_129191081.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-123)

Antitoxin

• Antitoxin ParD (3-65)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13469.30 Da
• Isoelectric Point: 7.3205

>T117973 WP_129191077.1 NZ_CP035491:2155077-2155448 [Agromyces protaetiae]
VTHRPERELVRGRVVLVELDPHRGAEQGKTRPAVIVSNDGANAAAARSDYGVITVVPLTSNVRTIRPYQALIPRDRSGLP
VDSKAQTEQVRSISTERVVNAIGWLDAELMAEVDEALRVHLSL

Nucleotide

• Length: 372 bp

>T117973 NZ_CP035491:2155077-2155448 [Agromyces protaetiae]
GTGACCCATCGTCCGGAGCGAGAGCTCGTGCGGGGGCGCGTCGTGCTCGTCGAACTCGATCCGCATCGCGGTGCGGAGCA
GGGGAAGACCCGACCGGCGGTGATCGTCAGCAATGATGGGGCAAATGCTGCCGCAGCGCGATCCGACTACGGCGTCATCA
CGGTTGTGCCGCTCACGAGCAACGTACGCACCATCCGGCCCTATCAAGCGTTGATTCCTCGTGACCGGAGTGGACTGCCC
GTCGACTCGAAGGCTCAGACCGAGCAGGTGAGAAGCATCTCGACCGAGCGCGTGGTGAACGCGATCGGGTGGCTCGACGC
CGAACTCATGGCCGAAGTCGATGAGGCGCTTCGCGTGCACCTATCGCTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8083.80 Da
• Isoelectric Point: 3.8964

>AT117973 WP_129191075.1 NZ_CP035491:2154856-2155080 [Agromyces protaetiae]
MKVSVSLDEADVAFLDADVERGVYESRSAAVAASIRLLRERELTQSYLEEFQEWGESGEADAWDAASGDGLVSK

Nucleotide

• Length: 225 bp

>AT117973 NZ_CP035491:2154856-2155080 [Agromyces protaetiae]
ATGAAGGTCAGCGTGAGTCTCGACGAGGCGGATGTTGCATTCCTTGACGCCGATGTAGAACGCGGCGTCTACGAGTCTCG
CTCAGCCGCAGTCGCGGCGTCCATCCGCTTGCTTCGGGAGCGCGAACTCACTCAGAGCTATCTCGAGGAGTTCCAGGAGT
GGGGCGAGTCGGGCGAGGCCGACGCGTGGGATGCTGCGTCGGGCGACGGACTGGTCTCCAAGTGA