Detailed information of TA system TA117969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4990328..4991148
• Replicon: chromosome
• Accession: NZ_CP035486
• Organism: Escherichia coli strain NissleGFP_pZE21

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: EUJ32_RS24375
• Protein ID: WP_001054379.1
• Coordinates: 4990891..4991148 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: EUJ32_RS24370
• Protein ID: WP_000123957.1
• Coordinates: 4990328..4990879 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EUJ32_RS24345	4986022..4987335	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
EUJ32_RS24350	4987347..4987625	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
EUJ32_RS24355	4987622..4988743	-	1122	WP_000010833.1	M42 family metallopeptidase	-
EUJ32_RS25045	4988988..4989104	-	117	Protein_4613	VOC family protein	-
EUJ32_RS24360	4989142..4989402	-	261	WP_000077645.1	hypothetical protein	-
EUJ32_RS24365	4989530..4990276	-	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
EUJ32_RS24370	4990328..4990879	-	552	WP_000123957.1	N-acetyltransferase	Antitoxin
EUJ32_RS24375	4990891..4991148	-	258	WP_001054379.1	YjhX family toxin	Toxin
EUJ32_RS24380	4991525..4991689	-	165	Protein_4618	GNAT family N-acetyltransferase	-
EUJ32_RS24390	4992932..4994038	+	1107	Protein_4620	helicase YjhR	-
EUJ32_RS24395	4994621..4995601	-	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T117969 WP_001054379.1 NZ_CP035486:c4991148-4990891 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T117969 NZ_CP035486:c4991148-4990891 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT117969 WP_000123957.1 NZ_CP035486:c4990879-4990328 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT117969 NZ_CP035486:c4990879-4990328 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030