Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3258465..3259119 Replicon chromosome
Accession NZ_CP035486
Organism Escherichia coli strain NissleGFP_pZE21

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag EUJ32_RS15860 Protein ID WP_000244781.1
Coordinates 3258465..3258872 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EUJ32_RS15865 Protein ID WP_000354046.1
Coordinates 3258853..3259119 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EUJ32_RS15840 3254422..3256155 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
EUJ32_RS15845 3256161..3256871 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EUJ32_RS15850 3256896..3257792 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EUJ32_RS15855 3257904..3258425 + 522 WP_001055874.1 flavodoxin FldB -
EUJ32_RS15860 3258465..3258872 - 408 WP_000244781.1 protein YgfX Toxin
EUJ32_RS15865 3258853..3259119 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EUJ32_RS15870 3259362..3260342 + 981 WP_000886079.1 tRNA-modifying protein YgfZ -
EUJ32_RS15875 3260419..3261078 - 660 WP_000250274.1 hemolysin III family protein -
EUJ32_RS15880 3261242..3261553 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
EUJ32_RS15885 3261598..3263031 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T117962 WP_000244781.1 NZ_CP035486:c3258872-3258465 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T117962 NZ_CP035486:c3258872-3258465 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT117962 WP_000354046.1 NZ_CP035486:c3259119-3258853 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT117962 NZ_CP035486:c3259119-3258853 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTTTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References