117948

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1226920..1227721 Replicon chromosome
Accession NZ_CP035486
Organism Escherichia coli strain NissleGFP_pZE21

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0H2V687
Locus tag EUJ32_RS05975 Protein ID WP_000854739.1
Coordinates 1227341..1227721 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P7R0L9
Locus tag EUJ32_RS05970 Protein ID WP_001285482.1
Coordinates 1226920..1227294 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EUJ32_RS05935 1223008..1223463 + 456 WP_000581502.1 hypothetical protein -
EUJ32_RS24975 1223582..1223793 - 212 Protein_1118 hypothetical protein -
EUJ32_RS05945 1223874..1224695 + 822 WP_001234710.1 DUF945 domain-containing protein -
EUJ32_RS05950 1224958..1225431 + 474 WP_000855076.1 antirestriction protein -
EUJ32_RS05955 1225447..1225923 + 477 WP_001186170.1 RadC family protein -
EUJ32_RS05960 1225986..1226207 + 222 WP_000692312.1 DUF987 domain-containing protein -
EUJ32_RS05965 1226226..1226870 + 645 WP_000094915.1 hypothetical protein -
EUJ32_RS05970 1226920..1227294 + 375 WP_001285482.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EUJ32_RS05975 1227341..1227721 + 381 WP_000854739.1 TA system toxin CbtA family protein Toxin
EUJ32_RS05980 1227718..1228206 + 489 WP_001054232.1 hypothetical protein -
EUJ32_RS05985 1228226..1228423 + 198 WP_000772027.1 DUF957 domain-containing protein -
EUJ32_RS05990 1228508..1229335 + 828 WP_001304477.1 DUF4942 domain-containing protein -
EUJ32_RS06000 1229822..1230760 + 939 WP_000351287.1 glyoxylate/hydroxypyruvate reductase GhrA -
EUJ32_RS06005 1230815..1231552 + 738 WP_000283664.1 zinc-binding phosphatase -
EUJ32_RS06010 1231576..1232130 + 555 WP_001001909.1 molecular chaperone YcdY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - csgG / csgF / csgE / csgD / csgB / csgA / csgC 1202152..1237663 35511


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 14214.14 Da        Isoelectric Point: 6.4773

>T117948 WP_000854739.1 NZ_CP035486:1227341-1227721 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNDITLGRHPEEAKQ

Download         Length: 381 bp

>T117948 NZ_CP035486:1227341-1227721 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCTGGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13791.44 Da        Isoelectric Point: 4.7511

>AT117948 WP_001285482.1 NZ_CP035486:1226920-1227294 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT117948 NZ_CP035486:1226920-1227294 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2V687

Antitoxin

Source ID Structure
AlphaFold DB A0A0P7R0L9

References