117946

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 288121..288919 Replicon chromosome
Accession NZ_CP035486
Organism Escherichia coli strain NissleGFP_pZE21

Toxin (Protein)


Gene name yeeV Uniprot ID Q8VRA6
Locus tag EUJ32_RS01370 Protein ID WP_000854730.1
Coordinates 288121..288498 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P0SQV8
Locus tag EUJ32_RS01375 Protein ID WP_001285481.1
Coordinates 288545..288919 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EUJ32_RS01340 283265..284669 + 1405 Protein_261 IS66 family transposase -
EUJ32_RS01345 284923..286056 - 1134 WP_000555401.1 IS110-like element ISEc45 family transposase -
EUJ32_RS01350 286117..286245 + 129 Protein_263 transposase domain-containing protein -
EUJ32_RS01355 286229..286417 + 189 Protein_264 IS66 family transposase -
EUJ32_RS01360 286565..287553 + 989 Protein_265 IS630 family transposase -
EUJ32_RS01365 287632..288124 - 493 Protein_266 hypothetical protein -
EUJ32_RS01370 288121..288498 - 378 WP_000854730.1 TA system toxin CbtA family protein Toxin
EUJ32_RS01375 288545..288919 - 375 WP_001285481.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EUJ32_RS01380 288969..289613 - 645 WP_000086759.1 hypothetical protein -
EUJ32_RS01385 289632..289853 - 222 WP_000692315.1 DUF987 domain-containing protein -
EUJ32_RS01390 289916..290392 - 477 WP_001313574.1 RadC family protein -
EUJ32_RS01395 290408..290881 - 474 WP_001313575.1 antirestriction protein -
EUJ32_RS01400 290975..291220 - 246 WP_001164966.1 hypothetical protein -
EUJ32_RS01405 291220..292041 - 822 WP_024199417.1 DUF945 domain-containing protein -
EUJ32_RS01410 292141..292374 - 234 WP_001213776.1 DUF905 family protein -
EUJ32_RS01415 292492..292944 - 453 WP_000682723.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14212.21 Da        Isoelectric Point: 7.2923

>T117946 WP_000854730.1 NZ_CP035486:c288498-288121 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Download         Length: 378 bp

>T117946 NZ_CP035486:c288498-288121 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13817.53 Da        Isoelectric Point: 4.7511

>AT117946 WP_001285481.1 NZ_CP035486:c288919-288545 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT117946 NZ_CP035486:c288919-288545 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2V671

Antitoxin

Source ID Structure
AlphaFold DB A0A0P0SQV8

References