Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 89124..89674 Replicon chromosome
Accession NZ_CP035486
Organism Escherichia coli strain NissleGFP_pZE21

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag EUJ32_RS00440 Protein ID WP_001160966.1
Coordinates 89360..89674 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag EUJ32_RS00435 Protein ID WP_000125566.1
Coordinates 89124..89357 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EUJ32_RS00410 84197..84484 + 288 WP_000203741.1 ferredoxin-like protein FixX -
EUJ32_RS00415 84543..85874 + 1332 WP_001183207.1 MFS transporter -
EUJ32_RS00420 85982..86512 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
EUJ32_RS00425 86505..88367 + 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
EUJ32_RS00430 88559..89038 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
EUJ32_RS00435 89124..89357 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
EUJ32_RS00440 89360..89674 + 315 WP_001160966.1 CcdB family protein Toxin
EUJ32_RS00445 89671..90519 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
EUJ32_RS00450 90526..90903 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
EUJ32_RS00455 90906..91727 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
EUJ32_RS00460 91724..92713 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
EUJ32_RS00465 92713..93999 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T117945 WP_001160966.1 NZ_CP035486:89360-89674 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T117945 NZ_CP035486:89360-89674 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT117945 WP_000125566.1 NZ_CP035486:89124-89357 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT117945 NZ_CP035486:89124-89357 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References