Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4922321..4923156 Replicon chromosome
Accession NZ_CP035477
Organism Escherichia coli strain U13A

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag U13A_RS24520 Protein ID WP_000854759.1
Coordinates 4922779..4923156 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag U13A_RS24515 Protein ID WP_001295723.1
Coordinates 4922321..4922689 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U13A_RS24480 4917449..4918195 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
U13A_RS24485 4918210..4919751 - 1542 WP_001298859.1 IS21-like element ISEc12 family transposase -
U13A_RS24490 4919947..4920567 + 621 Protein_4679 DUF932 domain-containing protein -
U13A_RS24500 4920909..4921382 + 474 WP_001350782.1 antirestriction protein -
U13A_RS24505 4921398..4921874 + 477 WP_001186775.1 RadC family protein -
U13A_RS24510 4921937..4922158 + 222 WP_000692345.1 DUF987 domain-containing protein -
U13A_RS24515 4922321..4922689 + 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
U13A_RS24520 4922779..4923156 + 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
U13A_RS26995 4923153..4923323 + 171 WP_158604577.1 hypothetical protein -
U13A_RS24525 4923338..4923763 + 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
U13A_RS24530 4923760..4924110 + 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
U13A_RS24535 4924141..4925743 + 1603 Protein_4688 IS66-like element ISEc23 family transposase -
U13A_RS24540 4925775..4926170 + 396 Protein_4689 hypothetical protein -
U13A_RS24545 4926187..4926363 + 177 WP_000839286.1 DUF957 domain-containing protein -
U13A_RS27000 4926463..4926621 + 159 WP_001467148.1 hypothetical protein -
U13A_RS24555 4926985..4927161 + 177 Protein_4692 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4926985..4948256 21271
- inside IScluster/Tn - - 4917449..4925743 8294


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T117926 WP_000854759.1 NZ_CP035477:4922779-4923156 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T117926 NZ_CP035477:4922779-4923156 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT117926 WP_001295723.1 NZ_CP035477:4922321-4922689 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT117926 NZ_CP035477:4922321-4922689 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References