Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2237476..2238307 Replicon chromosome
Accession NZ_CP035477
Organism Escherichia coli strain U13A

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag U13A_RS11485 Protein ID WP_000854815.1
Coordinates 2237933..2238307 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag U13A_RS11480 Protein ID WP_001280918.1
Coordinates 2237476..2237844 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U13A_RS11425 2232565..2233311 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
U13A_RS11435 2233394..2233744 + 351 Protein_2204 hypothetical protein -
U13A_RS11440 2233760..2234170 + 411 WP_000846703.1 hypothetical protein -
U13A_RS11450 2234391..2235209 + 819 WP_001542275.1 DUF945 domain-containing protein -
U13A_RS11455 2235209..2235454 + 246 WP_001164966.1 hypothetical protein -
U13A_RS11460 2235548..2236021 + 474 WP_001542276.1 antirestriction protein -
U13A_RS11465 2236037..2236513 + 477 WP_001186200.1 RadC family protein -
U13A_RS11470 2236576..2236797 + 222 WP_000692345.1 DUF987 domain-containing protein -
U13A_RS11475 2236816..2237460 + 645 WP_000086752.1 hypothetical protein -
U13A_RS11480 2237476..2237844 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
U13A_RS11485 2237933..2238307 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
U13A_RS11490 2238304..2238498 + 195 WP_000988600.1 hypothetical protein -
U13A_RS11495 2238511..2238624 + 114 WP_001161660.1 DUF957 domain-containing protein -
U13A_RS11500 2238913..2239059 - 147 Protein_2216 transposase domain-containing protein -
U13A_RS11505 2239113..2239295 + 183 WP_001296208.1 hypothetical protein -
U13A_RS11510 2239396..2239725 - 330 WP_000450409.1 DUF496 family protein -
U13A_RS11515 2239897..2240955 - 1059 WP_001200889.1 FUSC family protein -
U13A_RS11520 2241153..2241626 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
U13A_RS11525 2241745..2242911 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T117915 WP_000854815.1 NZ_CP035477:2237933-2238307 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T117915 NZ_CP035477:2237933-2238307 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT117915 WP_001280918.1 NZ_CP035477:2237476-2237844 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT117915 NZ_CP035477:2237476-2237844 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References