Detailed information of TA system TA117896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4896184..4897004
• Replicon: chromosome
• Accession: NZ_CP035476
• Organism: Escherichia coli strain U12A

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: U12A_RS26855
• Protein ID: WP_001054379.1
• Coordinates: 4896184..4896441 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: U12A_RS26860
• Protein ID: WP_000123957.1
• Coordinates: 4896453..4897004 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U12A_RS26840	4894046..4894300	-	255	WP_000788354.1	glycosyltransferase	-
U12A_RS26845	4894463..4895610	+	1148	WP_085949154.1	IS3-like element ISEc52 family transposase	-
U12A_RS26850	4895643..4895807	+	165	Protein_4654	GNAT family N-acetyltransferase	-
U12A_RS26855	4896184..4896441	+	258	WP_001054379.1	YjhX family toxin	Toxin
U12A_RS26860	4896453..4897004	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
U12A_RS26865	4897056..4897802	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
U12A_RS26870	4897930..4898190	+	261	WP_000077645.1	hypothetical protein	-
U12A_RS27805	4898228..4898344	+	117	Protein_4659	VOC family protein	-
U12A_RS26875	4898589..4899710	+	1122	WP_000010833.1	M42 family metallopeptidase	-
U12A_RS26880	4899707..4899985	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
U12A_RS26885	4899997..4901310	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4870928..4905225	34297

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T117896 WP_001054379.1 NZ_CP035476:4896184-4896441 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T117896 NZ_CP035476:4896184-4896441 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT117896 WP_000123957.1 NZ_CP035476:4896453-4897004 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT117896 NZ_CP035476:4896453-4897004 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030