Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4676472..4677304 Replicon chromosome
Accession NZ_CP035476
Organism Escherichia coli strain U12A

Toxin (Protein)


Gene name yeeV Uniprot ID S1PD64
Locus tag U12A_RS25650 Protein ID WP_000854753.1
Coordinates 4676472..4676846 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NQ68
Locus tag U12A_RS25655 Protein ID WP_001540478.1
Coordinates 4676936..4677304 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U12A_RS25625 4672049..4673587 - 1539 WP_001187191.1 lysine decarboxylation/transport transcriptional activator CadC -
U12A_RS25630 4673765..4674462 + 698 Protein_4429 IS1 family transposase -
U12A_RS25635 4675112..4675681 - 570 WP_129481423.1 DUF4942 domain-containing protein -
U12A_RS25640 4675778..4675975 - 198 WP_000839281.1 DUF957 domain-containing protein -
U12A_RS25645 4675987..4676475 - 489 WP_000777545.1 hypothetical protein -
U12A_RS25650 4676472..4676846 - 375 WP_000854753.1 TA system toxin CbtA family protein Toxin
U12A_RS25655 4676936..4677304 - 369 WP_001540478.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
U12A_RS25660 4677467..4677688 - 222 WP_000692311.1 DUF987 domain-containing protein -
U12A_RS25665 4677751..4678227 - 477 WP_001186786.1 RadC family protein -
U12A_RS25670 4678243..4678728 - 486 WP_000214398.1 antirestriction protein -
U12A_RS25675 4678819..4679637 - 819 WP_001773857.1 DUF945 domain-containing protein -
U12A_RS25680 4679727..4679960 - 234 WP_001278283.1 DUF905 family protein -
U12A_RS25685 4679966..4680643 - 678 WP_001097301.1 hypothetical protein -
U12A_RS25690 4680791..4681471 - 681 WP_001282919.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14005.00 Da        Isoelectric Point: 8.2905

>T117894 WP_000854753.1 NZ_CP035476:c4676846-4676472 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Download         Length: 375 bp

>T117894 NZ_CP035476:c4676846-4676472 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13652.53 Da        Isoelectric Point: 7.8398

>AT117894 WP_001540478.1 NZ_CP035476:c4677304-4676936 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT117894 NZ_CP035476:c4677304-4676936 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LVU0

Antitoxin

Source ID Structure
AlphaFold DB S1NQ68

References