Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3173808..3174462 Replicon chromosome
Accession NZ_CP035476
Organism Escherichia coli strain U12A

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag U12A_RS18270 Protein ID WP_000244765.1
Coordinates 3173808..3174215 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag U12A_RS18275 Protein ID WP_000354050.1
Coordinates 3174196..3174462 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U12A_RS18250 3169765..3171498 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
U12A_RS18255 3171504..3172214 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
U12A_RS18260 3172239..3173135 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
U12A_RS18265 3173247..3173768 + 522 WP_001055874.1 flavodoxin FldB -
U12A_RS18270 3173808..3174215 - 408 WP_000244765.1 protein YgfX Toxin
U12A_RS18275 3174196..3174462 - 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
U12A_RS18280 3174705..3175685 + 981 WP_000886084.1 tRNA-modifying protein YgfZ -
U12A_RS18285 3175762..3176421 - 660 WP_000250275.1 hemolysin III family protein -
U12A_RS18290 3176585..3176896 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
U12A_RS18295 3176941..3178374 + 1434 WP_001296350.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T117887 WP_000244765.1 NZ_CP035476:c3174215-3173808 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T117887 NZ_CP035476:c3174215-3173808 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT117887 WP_000354050.1 NZ_CP035476:c3174462-3174196 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT117887 NZ_CP035476:c3174462-3174196 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References