Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2237477..2238308 Replicon chromosome
Accession NZ_CP035476
Organism Escherichia coli strain U12A

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag U12A_RS14005 Protein ID WP_000854815.1
Coordinates 2237934..2238308 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag U12A_RS14000 Protein ID WP_001280918.1
Coordinates 2237477..2237845 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U12A_RS13945 2232566..2233312 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
U12A_RS13955 2233395..2233745 + 351 Protein_2203 hypothetical protein -
U12A_RS13960 2233761..2234171 + 411 WP_000846703.1 hypothetical protein -
U12A_RS13970 2234392..2235210 + 819 WP_001542275.1 DUF945 domain-containing protein -
U12A_RS13975 2235210..2235455 + 246 WP_001164966.1 hypothetical protein -
U12A_RS13980 2235549..2236022 + 474 WP_001542276.1 antirestriction protein -
U12A_RS13985 2236038..2236514 + 477 WP_001186200.1 RadC family protein -
U12A_RS13990 2236577..2236798 + 222 WP_000692345.1 DUF987 domain-containing protein -
U12A_RS13995 2236817..2237461 + 645 WP_000086752.1 hypothetical protein -
U12A_RS14000 2237477..2237845 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
U12A_RS14005 2237934..2238308 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
U12A_RS14010 2238305..2238499 + 195 WP_000988600.1 hypothetical protein -
U12A_RS14015 2238512..2238625 + 114 WP_001161660.1 DUF957 domain-containing protein -
U12A_RS14020 2238914..2239060 - 147 Protein_2215 transposase domain-containing protein -
U12A_RS14025 2239114..2239296 + 183 WP_001296208.1 hypothetical protein -
U12A_RS14030 2239397..2239726 - 330 WP_000450409.1 DUF496 family protein -
U12A_RS14035 2239898..2240956 - 1059 WP_001200889.1 FUSC family protein -
U12A_RS14040 2241154..2241627 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
U12A_RS14045 2241746..2242912 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T117885 WP_000854815.1 NZ_CP035476:2237934-2238308 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T117885 NZ_CP035476:2237934-2238308 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT117885 WP_001280918.1 NZ_CP035476:2237477-2237845 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT117885 NZ_CP035476:2237477-2237845 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References