Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yoeB-relB/Txe-YefM
Location 4711844..4712355 Replicon chromosome
Accession NZ_CP035467
Organism Methylotuvimicrobium buryatense strain 5GB1C

Toxin (Protein)


Gene name yoeB Uniprot ID A0A4P9USB6
Locus tag EQU24_RS20925 Protein ID WP_017841691.1
Coordinates 4712083..4712355 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G4SWS6
Locus tag EQU24_RS20920 Protein ID WP_014146801.1
Coordinates 4711844..4712086 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQU24_RS20880 4706909..4707205 + 297 WP_017841698.1 type II toxin-antitoxin system RelE/ParE family toxin -
EQU24_RS20885 4707214..4707513 + 300 WP_017841697.1 putative addiction module antidote protein -
EQU24_RS20890 4707940..4708251 - 312 WP_017841696.1 hypothetical protein -
EQU24_RS20895 4708351..4708614 - 264 WP_201764868.1 DUF2335 domain-containing protein -
EQU24_RS20900 4708571..4708813 - 243 WP_017841694.1 hypothetical protein -
EQU24_RS20905 4709466..4710749 + 1284 WP_017841684.1 ISKra4 family transposase -
EQU24_RS20910 4711022..4711375 - 354 WP_017841693.1 DUF5615 family PIN-like protein -
EQU24_RS20915 4711372..4711608 - 237 WP_017841692.1 DUF433 domain-containing protein -
EQU24_RS20920 4711844..4712086 + 243 WP_014146801.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EQU24_RS20925 4712083..4712355 + 273 WP_017841691.1 Txe/YoeB family addiction module toxin Toxin
EQU24_RS20930 4712579..4712833 + 255 WP_017841690.1 hypothetical protein -
EQU24_RS20935 4712833..4713129 + 297 WP_017841689.1 type II toxin-antitoxin system PemK/MazF family toxin -
EQU24_RS20940 4713500..4715245 + 1746 WP_017841687.1 2-oxoacid:acceptor oxidoreductase subunit alpha -
EQU24_RS20945 4715242..4716087 + 846 WP_017841686.1 2-oxoglutarate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(10-89)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10567.36 Da        Isoelectric Point: 10.3683

>T117849 WP_017841691.1 NZ_CP035467:4712083-4712355 [Methylotuvimicrobium buryatense]
VTFTAWKLVYTKQAQRDAKKLASSGLKLKAQELLELIAKDPYRKPPPFEKLIGDLTGAYSRRINIQHRLVYQVLEDERVV
KVLRLWSHYE

Download         Length: 273 bp

>T117849 NZ_CP035467:4712083-4712355 [Methylotuvimicrobium buryatense]
GTGACATTTACCGCATGGAAGTTGGTTTACACCAAGCAAGCTCAGAGGGATGCAAAGAAGTTGGCCTCCAGCGGCCTGAA
GCTAAAAGCACAGGAATTGCTGGAGCTGATAGCAAAAGATCCGTATCGTAAGCCGCCTCCGTTTGAGAAGCTCATCGGCG
ATCTTACGGGAGCCTATTCCCGCCGGATCAATATTCAGCATCGCCTAGTGTACCAAGTACTTGAAGATGAACGAGTAGTT
AAAGTTCTTCGGCTCTGGAGCCACTACGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9053.17 Da        Isoelectric Point: 4.2340

>AT117849 WP_014146801.1 NZ_CP035467:4711844-4712086 [Methylotuvimicrobium buryatense]
MTGITATEARSNLYRLIDETAESHQPIVITGKRNKAVLVSEEDWSAIQETLYLLSVPGMRESIRKGMDTPVDECNEELDW

Download         Length: 243 bp

>AT117849 NZ_CP035467:4711844-4712086 [Methylotuvimicrobium buryatense]
ATGACCGGAATAACTGCAACTGAGGCGCGCAGCAACCTTTATCGGTTGATTGATGAAACCGCCGAATCTCACCAACCAAT
CGTCATCACGGGTAAACGGAATAAAGCTGTTCTGGTTTCCGAAGAAGATTGGTCTGCAATTCAGGAAACGCTTTACCTGC
TATCCGTACCAGGCATGCGTGAGTCTATTCGGAAGGGTATGGATACTCCTGTGGATGAATGCAATGAAGAGCTCGACTGG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P9USB6

Antitoxin

Source ID Structure
AlphaFold DB G4SWS6

References