Detailed information of TA system TA117848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4706909..4707513
• Replicon: chromosome
• Accession: NZ_CP035467
• Organism: Methylotuvimicrobium buryatense strain 5GB1C

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A4P9UUM1
• Locus tag: EQU24_RS20880
• Protein ID: WP_017841698.1
• Coordinates: 4706909..4707205 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: EQU24_RS20885
• Protein ID: WP_017841697.1
• Coordinates: 4707214..4707513 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQU24_RS20865	4704582..4704830	+	249	WP_017841701.1	DUF2442 domain-containing protein	-
EQU24_RS20870	4704986..4705669	+	684	WP_017841700.1	NrdJb	-
EQU24_RS20875	4705804..4706517	+	714	WP_017841699.1	hypothetical protein	-
EQU24_RS20880	4706909..4707205	+	297	WP_017841698.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EQU24_RS20885	4707214..4707513	+	300	WP_017841697.1	putative addiction module antidote protein	Antitoxin
EQU24_RS20890	4707940..4708251	-	312	WP_017841696.1	hypothetical protein	-
EQU24_RS20895	4708351..4708614	-	264	WP_201764868.1	DUF2335 domain-containing protein	-
EQU24_RS20900	4708571..4708813	-	243	WP_017841694.1	hypothetical protein	-
EQU24_RS20905	4709466..4710749	+	1284	WP_017841684.1	ISKra4 family transposase	-
EQU24_RS20910	4711022..4711375	-	354	WP_017841693.1	DUF5615 family PIN-like protein	-
EQU24_RS20915	4711372..4711608	-	237	WP_017841692.1	DUF433 domain-containing protein	-
EQU24_RS20920	4711844..4712086	+	243	WP_014146801.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EQU24_RS20925	4712083..4712355	+	273	WP_017841691.1	Txe/YoeB family addiction module toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-93)

Antitoxin

• Uncharacterized protein HI1420 (6-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11020.66 Da
• Isoelectric Point: 10.3845

>T117848 WP_017841698.1 NZ_CP035467:4706909-4707205 [Methylotuvimicrobium buryatense]
MIEIRKTADFAKWLDNLHDLQARARVQTRIERLAAGNPGDVEPVGEGVSELRINYGPGYRVYFKKRGRELIILLAGGDKS
TQAKDIKTALRLARNLSE

Nucleotide

• Length: 297 bp

>T117848 NZ_CP035467:4706909-4707205 [Methylotuvimicrobium buryatense]
ATGATCGAAATTCGCAAAACCGCCGACTTCGCTAAATGGCTCGACAACCTGCATGATCTTCAAGCGAGAGCGCGCGTCCA
AACAAGGATCGAGCGGCTTGCCGCTGGAAACCCTGGAGATGTTGAACCCGTTGGCGAAGGTGTCTCGGAATTGCGAATCA
ATTATGGCCCCGGTTACCGGGTGTATTTCAAGAAGCGGGGGCGAGAACTGATCATTCTGCTGGCTGGCGGTGACAAGAGT
ACCCAAGCCAAGGATATTAAGACCGCCTTACGCCTCGCACGTAATCTTTCGGAATAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10633.13 Da
• Isoelectric Point: 6.7186

>AT117848 WP_017841697.1 NZ_CP035467:4707214-4707513 [Methylotuvimicrobium buryatense]
MTKTVTTRYDVAEHLRTPEEMAAYLEACLEEANGDAAFIAKALGDIARAKGMSQVARDAGLSRESLYKALSGERSPGFDT
ILKVIGALGLKLHAKAEHN

Nucleotide

• Length: 300 bp

>AT117848 NZ_CP035467:4707214-4707513 [Methylotuvimicrobium buryatense]
ATGACTAAGACTGTTACTACTCGCTATGACGTTGCCGAACACCTCAGAACACCAGAAGAAATGGCTGCTTATCTTGAGGC
ATGCCTTGAAGAAGCTAATGGGGATGCAGCATTCATTGCCAAAGCACTTGGTGACATAGCTCGGGCCAAAGGTATGTCAC
AGGTCGCGCGAGATGCAGGACTATCCCGTGAAAGCCTTTACAAGGCGCTTTCCGGTGAACGCAGCCCAGGCTTCGACACC
ATCCTTAAAGTTATCGGTGCGCTTGGGCTGAAGTTGCATGCCAAAGCCGAACACAATTGA

Structures

Toxin

• AlphaFold DB: A0A4P9UUM1