Detailed information of TA system TA117841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4001720..4002282
• Replicon: chromosome
• Accession: NZ_CP035467
• Organism: Methylotuvimicrobium buryatense strain 5GB1C

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: EQU24_RS17890
• Protein ID: WP_201764874.1
• Coordinates: 4001720..4001995 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A4P9UR17
• Locus tag: EQU24_RS17895
• Protein ID: WP_017842250.1
• Coordinates: 4002037..4002282 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQU24_RS17880	3997667..3999586	+	1920	WP_017842253.1	molecular chaperone HtpG	-
EQU24_RS17885	4000102..4001427	+	1326	WP_017840197.1	IS110 family transposase	-
EQU24_RS17890	4001720..4001995	-	276	WP_201764874.1	CcdB family protein	Toxin
EQU24_RS17895	4002037..4002282	-	246	WP_017842250.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
EQU24_RS17900	4002448..4003296	-	849	WP_017842249.1	polyamine aminopropyltransferase	-
EQU24_RS17910	4003906..4005849	+	1944	WP_083877868.1	biosynthetic arginine decarboxylase	-
EQU24_RS17915	4005880..4006065	+	186	WP_017842246.1	hypothetical protein	-
EQU24_RS17920	4006053..4006367	+	315	WP_017842245.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EQU24_RS17925	4006369..4007235	+	867	WP_017842244.1	NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-90)

Antitoxin

• Post-segregation antitoxin CcdA (11-81)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10141.02 Da
• Isoelectric Point: 6.1918

>T117841 WP_201764874.1 NZ_CP035467:c4001995-4001720 [Methylotuvimicrobium buryatense]
MKAYPYLLDIQSNLLDELRTTVVIPLCPTNLAGKAAITKLCPILKIDDRSFIVLTQQIAGIDRKYLGKEICDLSQYRSEI
IAALDFIISGI

Nucleotide

• Length: 276 bp

>T117841 NZ_CP035467:c4001995-4001720 [Methylotuvimicrobium buryatense]
ATGAAAGCCTACCCTTACCTTTTGGATATCCAATCCAACTTACTTGACGAGTTACGGACCACAGTCGTCATTCCGCTTTG
CCCTACTAACCTTGCAGGTAAAGCTGCAATTACAAAGCTATGTCCAATCTTAAAAATTGATGACAGATCTTTTATTGTGC
TCACCCAGCAAATTGCCGGCATAGATCGAAAATATCTTGGTAAAGAAATTTGCGACCTTTCGCAGTATCGCTCTGAAATT
ATTGCCGCATTGGACTTCATCATTTCCGGCATTTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9197.45 Da
• Isoelectric Point: 5.0494

>AT117841 WP_017842250.1 NZ_CP035467:c4002282-4002037 [Methylotuvimicrobium buryatense]
MPQVFDTHAPKKPTNVSINSDLLVKAKELKINLSATLETALTELVNARQRELWLCENKIAIEAYNQLVEDHGNFSDDLRS
F

Nucleotide

• Length: 246 bp

>AT117841 NZ_CP035467:c4002282-4002037 [Methylotuvimicrobium buryatense]
ATGCCCCAGGTCTTTGACACTCACGCCCCAAAAAAACCAACAAACGTCAGTATCAACAGTGATTTACTTGTTAAAGCGAA
AGAACTAAAGATCAATTTGTCGGCAACCTTGGAAACAGCTTTAACCGAATTAGTAAACGCTCGGCAACGAGAACTTTGGC
TGTGTGAAAACAAGATTGCCATCGAAGCATACAATCAATTGGTAGAGGATCATGGAAACTTTAGCGACGATTTGAGGAGT
TTTTAG

Structures

Antitoxin

• AlphaFold DB: A0A4P9UR17