Detailed information of TA system TA117839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3143311..3143948
• Replicon: chromosome
• Accession: NZ_CP035467
• Organism: Methylotuvimicrobium buryatense strain 5GB1C

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EQU24_RS14055
• Protein ID: WP_209005073.1
• Coordinates: 3143556..3143948 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: A0A4P9UPB3
• Locus tag: EQU24_RS14050
• Protein ID: WP_017838893.1
• Coordinates: 3143311..3143541 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQU24_RS14040	3139207..3140199	-	993	WP_017838895.1	hypothetical protein	-
EQU24_RS14045	3140266..3143232	-	2967	WP_017838894.1	CRISPR-associated endonuclease Cas3''	-
EQU24_RS14050	3143311..3143541	+	231	WP_017838893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EQU24_RS14055	3143556..3143948	+	393	WP_209005073.1	PIN domain-containing protein	Toxin
EQU24_RS14060	3144005..3144952	-	948	WP_017838891.1	type I-F CRISPR-associated endonuclease Cas1	-
EQU24_RS14065	3145320..3146186	+	867	WP_017838889.1	WYL domain-containing protein	-
EQU24_RS14070	3146232..3146804	+	573	WP_017838888.1	DNA-3-methyladenine glycosylase I	-
EQU24_RS14075	3147330..3148196	-	867	WP_017838887.1	RNA polymerase sigma factor RpoH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-122)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14769.27 Da
• Isoelectric Point: 7.7492

>T117839 WP_209005073.1 NZ_CP035467:3143556-3143948 [Methylotuvimicrobium buryatense]
MLDTDICIYLKKRRPACIAERFRQLRHGEVVISMITYGELFSGALKSREKEAAIMNVQLLAARLPVQPMLVEVAEVYASI
RSDLERKGCLIGGNDLWIAAHAVALDLTLVTNNTREFRRIDGLRLENWVE

Nucleotide

• Length: 393 bp

>T117839 NZ_CP035467:3143556-3143948 [Methylotuvimicrobium buryatense]
ATGCTGGATACGGATATTTGTATTTATCTTAAAAAACGCCGTCCGGCTTGCATAGCAGAGAGGTTCAGGCAATTGCGGCA
TGGCGAGGTAGTGATTTCGATGATTACCTATGGTGAGTTGTTTTCGGGCGCATTGAAAAGCCGAGAAAAAGAAGCGGCCA
TCATGAATGTCCAACTGCTGGCTGCTCGTTTGCCGGTGCAACCTATGCTGGTTGAGGTTGCGGAGGTTTATGCTTCTATT
CGCAGTGACTTGGAAAGAAAAGGTTGTCTGATTGGGGGCAATGACCTTTGGATCGCCGCTCACGCAGTTGCTTTGGATTT
GACATTGGTCACTAATAACACAAGGGAGTTTAGACGTATTGATGGCTTGCGGCTTGAAAATTGGGTTGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8826.89 Da
• Isoelectric Point: 4.3730

>AT117839 WP_017838893.1 NZ_CP035467:3143311-3143541 [Methylotuvimicrobium buryatense]
MTTTRVFKSGNSQAVRIPKEFQFDADEVEIFRRGDEVVLRKKPDNLADVMDIFAGFSDDFMAEKRIDPPPQDRDFE

Nucleotide

• Length: 231 bp

>AT117839 NZ_CP035467:3143311-3143541 [Methylotuvimicrobium buryatense]
ATGACGACAACGCGCGTTTTTAAAAGCGGCAATTCGCAAGCGGTGCGGATTCCCAAAGAATTCCAGTTTGATGCGGATGA
GGTAGAGATTTTTCGACGCGGAGATGAGGTAGTATTACGCAAAAAACCGGATAATCTTGCGGATGTGATGGACATTTTCG
CCGGCTTCTCAGATGATTTTATGGCGGAAAAACGGATTGACCCTCCACCACAGGATCGGGATTTTGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A4P9UPB3