Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2877456..2878040 Replicon chromosome
Accession NZ_CP035467
Organism Methylotuvimicrobium buryatense strain 5GB1C

Toxin (Protein)


Gene name hicA Uniprot ID A0A4P9UNQ6
Locus tag EQU24_RS12820 Protein ID WP_017839128.1
Coordinates 2877456..2877638 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A4P9UME7
Locus tag EQU24_RS12825 Protein ID WP_017839127.1
Coordinates 2877648..2878040 (+) Length 131 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQU24_RS12810 2874354..2875532 + 1179 WP_017839828.1 group II intron reverse transcriptase/maturase -
EQU24_RS12815 2875911..2876903 - 993 WP_138767173.1 cytosolic protein -
EQU24_RS12820 2877456..2877638 + 183 WP_017839128.1 type II toxin-antitoxin system HicA family toxin Toxin
EQU24_RS12825 2877648..2878040 + 393 WP_017839127.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EQU24_RS12830 2878372..2878833 - 462 WP_083877599.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
EQU24_RS12835 2878945..2879517 + 573 WP_017839125.1 GspH/FimT family pseudopilin -
EQU24_RS12840 2879552..2880061 + 510 WP_017839124.1 GspH/FimT family protein -
EQU24_RS12845 2880079..2880837 + 759 WP_017839123.1 hypothetical protein -
EQU24_RS12850 2880860..2882143 + 1284 WP_017839122.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-125)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6724.75 Da        Isoelectric Point: 10.5730

>T117838 WP_017839128.1 NZ_CP035467:2877456-2877638 [Methylotuvimicrobium buryatense]
MDSKQLIKQLEADGWILARIKGSHHQFKHPDKPGLVTVKHPDSDIPAGTLNSIRKQAGWK

Download         Length: 183 bp

>T117838 NZ_CP035467:2877456-2877638 [Methylotuvimicrobium buryatense]
ATGGACAGTAAACAACTCATCAAGCAACTTGAAGCCGACGGCTGGATTCTGGCACGAATCAAAGGCTCACATCACCAGTT
CAAGCACCCGGATAAACCCGGCTTGGTGACGGTCAAACATCCTGACAGCGACATTCCCGCCGGTACGCTGAACAGCATTC
GCAAACAGGCGGGATGGAAATAA

Antitoxin


Download         Length: 131 a.a.        Molecular weight: 14000.87 Da        Isoelectric Point: 4.5844

>AT117838 WP_017839127.1 NZ_CP035467:2877648-2878040 [Methylotuvimicrobium buryatense]
MRFSVVLHTDDGVRYGVTVPDLPGCFSAGDTLDDALASVAEAIDCHIETLIEDGADIPDCKPIAEHLSNPDYSGGVWAFV
DVPVEKYFGPAEKINITLPRLLLARIDRYAKAHGATRSGFLAEAARAAMR

Download         Length: 393 bp

>AT117838 NZ_CP035467:2877648-2878040 [Methylotuvimicrobium buryatense]
ATGCGCTTTTCTGTAGTATTGCATACCGATGACGGCGTTCGTTACGGCGTTACCGTCCCCGACTTGCCCGGCTGTTTTTC
GGCGGGCGACACCCTTGACGATGCATTGGCTTCCGTTGCTGAAGCGATTGATTGTCACATTGAAACATTGATTGAAGACG
GAGCCGATATTCCTGATTGCAAACCGATTGCCGAGCACCTATCCAATCCGGATTATTCAGGCGGCGTTTGGGCTTTCGTC
GACGTTCCGGTTGAAAAATACTTCGGCCCCGCCGAGAAAATCAACATCACGCTGCCGCGCCTGTTGTTGGCGAGAATCGA
TCGCTATGCGAAAGCGCACGGCGCAACGCGCTCCGGTTTTTTGGCTGAGGCGGCTCGTGCGGCAATGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P9UNQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A4P9UME7

References