Detailed information of TA system TA117837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 2423601..2424081
• Replicon: chromosome
• Accession: NZ_CP035467
• Organism: Methylotuvimicrobium buryatense strain 5GB1C

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: EQU24_RS10905
• Protein ID: WP_201764885.1
• Coordinates: 2423860..2424081 (-)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A4P9USI2
• Locus tag: EQU24_RS10900
• Protein ID: WP_017839475.1
• Coordinates: 2423601..2423870 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQU24_RS10890	2419802..2420545	-	744	WP_017839477.1	type I-C CRISPR-associated protein Cas5	-
EQU24_RS10895	2420555..2423014	-	2460	WP_201764886.1	CRISPR-associated endonuclease Cas3''	-
EQU24_RS10900	2423601..2423870	-	270	WP_017839475.1	hypothetical protein	Antitoxin
EQU24_RS10905	2423860..2424081	-	222	WP_201764885.1	BrnT family toxin	Toxin
EQU24_RS10910	2425002..2426327	-	1326	WP_017839472.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2420555..2464462	43907

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (2-67)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8612.82 Da
• Isoelectric Point: 8.6219

>T117837 WP_201764885.1 NZ_CP035467:c2424081-2423860 [Methylotuvimicrobium buryatense]
MEKHGIDFLDAQALWRDTDLLELQARSDDEPRFLVIGLIGSKHWSAVVTYRNGTVRLISVRRSRKREVELYER

Nucleotide

• Length: 222 bp

>T117837 NZ_CP035467:c2424081-2423860 [Methylotuvimicrobium buryatense]
CTGGAAAAGCACGGAATCGACTTTCTGGATGCCCAGGCCCTCTGGCGAGACACTGACCTGCTGGAACTCCAAGCTAGATC
GGATGATGAACCTCGTTTTCTGGTCATAGGTCTGATTGGTTCGAAACACTGGTCAGCCGTCGTCACCTATAGAAACGGAA
CAGTTCGGCTTATTTCTGTAAGGCGCTCCCGCAAACGAGAGGTAGAACTATATGAACGCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10071.37 Da
• Isoelectric Point: 8.6800

>AT117837 WP_017839475.1 NZ_CP035467:c2423870-2423601 [Methylotuvimicrobium buryatense]
MNAKDFDKKFDQGKKDIIDDLDLFTARRVNQEQKRINVDFPSWVVDSLDREAARIGVTRQSIIKVWLVERLKAEAANKAL
NGDASSGGH

Nucleotide

• Length: 270 bp

>AT117837 NZ_CP035467:c2423870-2423601 [Methylotuvimicrobium buryatense]
ATGAACGCTAAAGATTTTGATAAGAAATTTGACCAAGGCAAAAAAGACATCATTGATGATCTTGACTTGTTTACGGCGCG
TCGTGTCAACCAGGAACAGAAGCGCATAAATGTCGACTTTCCTTCGTGGGTAGTAGACTCGTTGGACCGTGAAGCGGCTC
GTATCGGTGTTACCCGGCAATCGATCATCAAGGTCTGGCTGGTTGAGCGCTTGAAAGCTGAAGCTGCTAACAAAGCCCTC
AATGGTGACGCCTCAAGCGGCGGACATTAG

Structures

Antitoxin

• AlphaFold DB: A0A4P9USI2