Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1961614..1962198 Replicon chromosome
Accession NZ_CP035467
Organism Methylotuvimicrobium buryatense strain 5GB1C

Toxin (Protein)


Gene name hicA Uniprot ID A0A4P9UNQ6
Locus tag EQU24_RS08850 Protein ID WP_017839128.1
Coordinates 1962016..1962198 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A4P9UME7
Locus tag EQU24_RS08845 Protein ID WP_017839127.1
Coordinates 1961614..1962006 (-) Length 131 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQU24_RS08825 1957150..1958151 + 1002 WP_017839836.1 NAD-dependent epimerase/dehydratase family protein -
EQU24_RS08830 1958455..1959087 - 633 WP_175421673.1 transposase -
EQU24_RS08835 1959181..1960359 + 1179 WP_138767139.1 ISAzo13 family transposase -
EQU24_RS08840 1960370..1961011 - 642 WP_138767140.1 transposase -
EQU24_RS22360 1961037..1961210 - 174 WP_017839982.1 hypothetical protein -
EQU24_RS08845 1961614..1962006 - 393 WP_017839127.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EQU24_RS08850 1962016..1962198 - 183 WP_017839128.1 type II toxin-antitoxin system HicA family toxin Toxin
EQU24_RS08855 1962418..1963410 + 993 WP_017839829.1 hypothetical protein -
EQU24_RS08860 1963789..1964967 - 1179 WP_017839828.1 group II intron reverse transcriptase/maturase -
EQU24_RS08865 1966029..1966589 + 561 WP_017839827.1 Uma2 family endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-125)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6724.75 Da        Isoelectric Point: 10.5730

>T117835 WP_017839128.1 NZ_CP035467:c1962198-1962016 [Methylotuvimicrobium buryatense]
MDSKQLIKQLEADGWILARIKGSHHQFKHPDKPGLVTVKHPDSDIPAGTLNSIRKQAGWK

Download         Length: 183 bp

>T117835 NZ_CP035467:c1962198-1962016 [Methylotuvimicrobium buryatense]
ATGGACAGTAAACAACTCATCAAGCAACTTGAAGCCGACGGCTGGATTCTGGCACGAATCAAAGGCTCACATCACCAGTT
CAAGCACCCGGATAAACCCGGCTTGGTGACGGTCAAACATCCTGACAGCGACATTCCCGCCGGTACGCTGAACAGCATTC
GCAAACAGGCGGGATGGAAATAA

Antitoxin


Download         Length: 131 a.a.        Molecular weight: 14000.87 Da        Isoelectric Point: 4.5844

>AT117835 WP_017839127.1 NZ_CP035467:c1962006-1961614 [Methylotuvimicrobium buryatense]
MRFSVVLHTDDGVRYGVTVPDLPGCFSAGDTLDDALASVAEAIDCHIETLIEDGADIPDCKPIAEHLSNPDYSGGVWAFV
DVPVEKYFGPAEKINITLPRLLLARIDRYAKAHGATRSGFLAEAARAAMR

Download         Length: 393 bp

>AT117835 NZ_CP035467:c1962006-1961614 [Methylotuvimicrobium buryatense]
ATGCGCTTTTCTGTAGTATTGCATACCGATGACGGCGTTCGTTACGGCGTTACCGTCCCCGACTTGCCCGGCTGTTTTTC
GGCGGGCGACACCCTTGACGATGCATTGGCTTCCGTTGCTGAAGCGATTGATTGTCACATTGAAACATTGATTGAAGACG
GAGCCGATATTCCTGATTGCAAACCGATTGCCGAGCACCTATCCAATCCGGATTATTCAGGCGGCGTTTGGGCTTTCGTC
GACGTTCCGGTTGAAAAATACTTCGGCCCCGCCGAGAAAATCAACATCACGCTGCCGCGCCTGTTGTTGGCGAGAATCGA
TCGCTATGCGAAAGCGCACGGCGCAACGCGCTCCGGTTTTTTGGCTGAGGCGGCTCGTGCGGCAATGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P9UNQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A4P9UME7

References