Detailed information of TA system TA117831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 410293..410954
• Replicon: chromosome
• Accession: NZ_CP035467
• Organism: Methylotuvimicrobium buryatense strain 5GB1C

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EQU24_RS01935
• Protein ID: WP_017841122.1
• Coordinates: 410541..410954 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4P9UJG8
• Locus tag: EQU24_RS01930
• Protein ID: WP_017841123.1
• Coordinates: 410293..410544 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQU24_RS01920	407429..408643	+	1215	WP_017841125.1	PepSY domain-containing protein	-
EQU24_RS01925	408817..410052	+	1236	WP_051056279.1	PepSY domain-containing protein	-
EQU24_RS01930	410293..410544	+	252	WP_017841123.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EQU24_RS01935	410541..410954	+	414	WP_017841122.1	type II toxin-antitoxin system VapC family toxin	Toxin
EQU24_RS01940	411458..411733	+	276	WP_017841120.1	hypothetical protein	-
EQU24_RS01945	412108..412887	+	780	WP_017841119.1	DUF3450 domain-containing protein	-
EQU24_RS01950	412884..414266	+	1383	WP_017841118.1	MotA/TolQ/ExbB proton channel family protein	-
EQU24_RS01955	414250..414768	+	519	WP_017841117.1	MotA/TolQ/ExbB proton channel family protein	-
EQU24_RS01960	414885..415289	+	405	WP_017841116.1	biopolymer transporter ExbD	-
EQU24_RS01965	415286..415915	+	630	WP_017841115.1	energy transducer TonB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-62)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15739.05 Da
• Isoelectric Point: 4.9464

>T117831 WP_017841122.1 NZ_CP035467:410541-410954 [Methylotuvimicrobium buryatense]
MSYLVDTNVLSELRRREPNQGVIEWFEQRPAGTLYLSVLTLGEIRKGIDLLNNDDRQLKLLDWLEVELPQFFSGRILSVD
ADVADRWGRLIAQIGRPIPAIDSLIAATALRHGLKLVTRNVRDFSYPELDVINPWRD

Nucleotide

• Length: 414 bp

>T117831 NZ_CP035467:410541-410954 [Methylotuvimicrobium buryatense]
ATGAGTTATCTGGTCGATACCAACGTGTTATCGGAATTGCGTCGCCGTGAACCCAATCAGGGCGTCATCGAATGGTTCGA
ACAACGTCCAGCCGGCACGCTTTACCTCAGCGTACTAACCTTGGGAGAAATCCGTAAAGGTATCGATTTGCTGAACAATG
ACGATCGACAGCTCAAACTACTGGATTGGCTGGAAGTAGAACTGCCCCAGTTTTTTTCCGGTCGGATTTTGAGCGTGGAT
GCTGATGTAGCTGACCGCTGGGGACGATTGATTGCTCAAATAGGGCGTCCCATTCCAGCGATCGACAGCCTGATTGCGGC
TACAGCGCTTAGGCATGGATTGAAGCTGGTTACCCGCAATGTTCGCGACTTCAGTTACCCTGAGCTCGATGTCATCAATC
CCTGGCGAGATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9681.85 Da
• Isoelectric Point: 6.2215

>AT117831 WP_017841123.1 NZ_CP035467:410293-410544 [Methylotuvimicrobium buryatense]
MHKWQIQEAKARLSTVLRSAEQDGPQDITHHGRSVAVLLSRADYDRLTGNRQSLVEFMRRSPLYDQDDLIIERDASLTRE
FEL

Nucleotide

• Length: 252 bp

>AT117831 NZ_CP035467:410293-410544 [Methylotuvimicrobium buryatense]
ATGCATAAATGGCAAATTCAGGAGGCGAAAGCGCGTCTTTCCACCGTATTACGCAGCGCCGAACAGGATGGGCCTCAGGA
CATCACCCATCACGGTCGTTCTGTTGCGGTACTGCTCTCACGCGCCGATTACGACCGACTGACGGGTAATCGGCAGTCGC
TGGTTGAATTCATGCGTCGTTCGCCACTTTATGATCAGGACGATCTCATCATAGAACGCGATGCCAGTCTGACCCGTGAA
TTCGAACTATGA

Structures

Antitoxin

• AlphaFold DB: A0A4P9UJG8