Detailed information of TA system TA117830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 47307..47746
• Replicon: chromosome
• Accession: NZ_CP035467
• Organism: Methylotuvimicrobium buryatense strain 5GB1C

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EQU24_RS00220
• Protein ID: WP_083877746.1
• Coordinates: 47519..47746 (+)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4P9UI96
• Locus tag: EQU24_RS00215
• Protein ID: WP_017841435.1
• Coordinates: 47307..47516 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQU24_RS00190	42657..42884	-	228	WP_138767047.1	hypothetical protein	-
EQU24_RS00195	42875..43876	-	1002	WP_017841438.1	DUF4338 domain-containing protein	-
EQU24_RS00200	44198..44386	+	189	WP_175421645.1	hypothetical protein	-
EQU24_RS00205	44527..46077	-	1551	WP_138767258.1	IS66 family transposase	-
EQU24_RS00210	46178..47107	+	930	WP_017841436.1	DUF3800 domain-containing protein	-
EQU24_RS00215	47307..47516	+	210	WP_017841435.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EQU24_RS00220	47519..47746	+	228	WP_083877746.1	type II toxin-antitoxin system HicA family toxin	Toxin
EQU24_RS00225	48210..48518	-	309	WP_017841434.1	HAD hydrolase-like protein	-
EQU24_RS00230	48729..48950	+	222	WP_014146562.1	hypothetical protein	-
EQU24_RS00235	48953..49252	+	300	WP_017841433.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EQU24_RS00245	49502..50443	-	942	WP_017841432.1	cation diffusion facilitator family transporter	-
EQU24_RS00250	50779..51069	+	291	WP_017841431.1	hypothetical protein	-
EQU24_RS00255	51192..51482	+	291	WP_017841430.1	hypothetical protein	-
EQU24_RS00260	51604..51894	+	291	WP_017841429.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (10-65)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8417.98 Da
• Isoelectric Point: 10.7496

>T117830 WP_083877746.1 NZ_CP035467:47519-47746 [Methylotuvimicrobium buryatense]
MGNIPVLKPREVVALLEILGFTEIRQRGSHKQFRHPDGRCTTVPFHAGRDISPVLLRQIAKDIGMTVGELLKFLV

Nucleotide

• Length: 228 bp

>T117830 NZ_CP035467:47519-47746 [Methylotuvimicrobium buryatense]
ATGGGAAACATACCAGTTCTTAAACCTCGCGAAGTTGTGGCTTTGCTTGAAATATTAGGATTTACAGAAATCCGGCAACG
CGGCTCACATAAACAATTCCGTCATCCTGACGGTAGATGCACAACAGTGCCATTTCATGCAGGACGTGATATCTCGCCAG
TTTTACTTCGGCAAATCGCTAAGGATATTGGCATGACAGTTGGAGAGTTACTTAAATTTCTAGTCTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7628.62 Da
• Isoelectric Point: 3.9484

>AT117830 WP_017841435.1 NZ_CP035467:47307-47516 [Methylotuvimicrobium buryatense]
MKNYTAIIEKCRDTGLYVGFIPGFTGAHSQAESLDELNQNLREVIEMLLEDGEPILEAEFVGTQNVVVA

Nucleotide

• Length: 210 bp

>AT117830 NZ_CP035467:47307-47516 [Methylotuvimicrobium buryatense]
ATGAAAAATTACACGGCTATTATTGAAAAATGTCGCGATACCGGATTATACGTGGGCTTCATTCCTGGATTTACAGGTGC
ACATAGCCAAGCGGAATCATTGGATGAGCTTAATCAAAATCTTCGGGAAGTCATTGAAATGTTACTTGAGGATGGCGAGC
CAATACTGGAAGCCGAGTTTGTTGGAACTCAAAATGTAGTTGTTGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A4P9UI96