Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 921635..922221 Replicon chromosome
Accession NZ_CP035466
Organism Klebsiella aerogenes strain LU2

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag ES159_RS04660 Protein ID WP_002920800.1
Coordinates 921635..922003 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag ES159_RS04665 Protein ID WP_004174006.1
Coordinates 922000..922221 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ES159_RS04640 917119..918189 - 1071 WP_049035288.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
ES159_RS04645 918191..919036 - 846 WP_015369329.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
ES159_RS04650 919033..919920 - 888 WP_045375777.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
ES159_RS04655 920027..921343 - 1317 WP_049035282.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
ES159_RS04660 921635..922003 - 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
ES159_RS04665 922000..922221 - 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ES159_RS04670 922385..923098 - 714 WP_086538103.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
ES159_RS04675 923116..923883 - 768 WP_015369325.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
ES159_RS04680 923880..925157 - 1278 WP_015369324.1 branched chain amino acid ABC transporter permease LivM -
ES159_RS04685 925154..926080 - 927 WP_015369323.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 916382..932481 16099


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T117818 WP_002920800.1 NZ_CP035466:c922003-921635 [Klebsiella aerogenes]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T117818 NZ_CP035466:c922003-921635 [Klebsiella aerogenes]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT117818 WP_004174006.1 NZ_CP035466:c922221-922000 [Klebsiella aerogenes]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT117818 NZ_CP035466:c922221-922000 [Klebsiella aerogenes]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References