Detailed information of TA system TA117816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1482216..1482814
• Replicon: chromosome
• Accession: NZ_CP035464
• Organism: Bifidobacterium pullorum subsp. gallinarum strain CACC 514

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: ESN35_RS06330
• Protein ID: WP_129237514.1
• Coordinates: 1482216..1482533 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ESN35_RS06335
• Protein ID: WP_129237516.1
• Coordinates: 1482527..1482814 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ESN35_RS06295	1477567..1477971	-	405	WP_129237503.1	pyridoxamine 5'-phosphate oxidase family protein	-
ESN35_RS06300	1478102..1479103	-	1002	WP_129238346.1	VanZ family protein	-
ESN35_RS06305	1479358..1480041	-	684	WP_129237504.1	hypothetical protein	-
ESN35_RS06310	1480235..1480816	+	582	WP_129237506.1	GNAT family N-acetyltransferase	-
ESN35_RS06315	1480832..1481026	-	195	WP_205730434.1	helix-turn-helix domain-containing protein	-
ESN35_RS06320	1481084..1481293	-	210	WP_129237510.1	hypothetical protein	-
ESN35_RS06325	1481624..1482049	+	426	WP_129237512.1	hypothetical protein	-
ESN35_RS06330	1482216..1482533	+	318	WP_129237514.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ESN35_RS06335	1482527..1482814	+	288	WP_129237516.1	putative addiction module antidote protein	Antitoxin
ESN35_RS06340	1482849..1483475	-	627	WP_129237518.1	hypothetical protein	-
ESN35_RS06345	1483974..1485113	+	1140	WP_129237519.1	IS1249 family transposase	-
ESN35_RS06360	1485607..1486638	-	1032	WP_129237521.1	hypothetical protein	-
ESN35_RS06365	1486888..1487658	+	771	WP_129237523.1	DUF1287 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1484010..1485113	1103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-94)

Antitoxin

• Uncharacterized protein HI1420 (4-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12166.87 Da
• Isoelectric Point: 10.1681

>T117816 WP_129237514.1 NZ_CP035464:1482216-1482533 [Bifidobacterium pullorum subsp. gallinarum]
VVTEIRKTDEYDAWFRDLRDRQAKARINARILLWQTAGRPVGDINTVGDGVSELRFRFGPGYRVYFAQKGDVVMLLLAGG
DKRTQSKDIDRARHMLAELKEDKQW

Nucleotide

• Length: 318 bp

>T117816 NZ_CP035464:1482216-1482533 [Bifidobacterium pullorum subsp. gallinarum]
GTGGTCACGGAGATACGCAAGACCGACGAGTACGACGCCTGGTTCCGCGACCTGCGGGATCGGCAGGCCAAGGCGCGGAT
CAACGCGCGGATCCTTCTGTGGCAGACGGCTGGAAGACCCGTGGGCGACATCAACACCGTCGGGGACGGGGTCAGCGAAC
TGCGGTTCCGCTTCGGCCCCGGCTACCGCGTCTACTTCGCGCAGAAGGGGGACGTGGTCATGCTGCTGCTCGCGGGCGGA
GACAAACGCACACAGTCCAAGGACATCGACAGGGCCAGGCACATGCTGGCCGAACTCAAGGAGGACAAGCAATGGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 9964.26 Da
• Isoelectric Point: 4.4230

>AT117816 WP_129237516.1 NZ_CP035464:1482527-1482814 [Bifidobacterium pullorum subsp. gallinarum]
MVTTSVWDASEYLEDEQDAIAYINAAAELDDPALLQAAIGDVAKARGMGQIAKEAGVGRESLYKSLGRDGNPSFRTIAKV
VKALGGRIVIEPSAA

Nucleotide

• Length: 288 bp

>AT117816 NZ_CP035464:1482527-1482814 [Bifidobacterium pullorum subsp. gallinarum]
ATGGTAACGACAAGCGTCTGGGACGCCAGCGAATACCTCGAGGACGAGCAGGACGCCATCGCCTACATCAACGCCGCGGC
CGAACTCGACGACCCCGCCTTGCTCCAGGCCGCCATCGGAGACGTGGCCAAGGCCCGCGGCATGGGCCAGATCGCCAAGG
AGGCCGGCGTCGGACGAGAAAGCCTGTACAAGAGCCTCGGCCGCGACGGCAACCCGTCGTTCCGTACCATCGCCAAGGTC
GTCAAAGCCCTCGGCGGCCGCATCGTCATCGAACCATCGGCCGCATGA