Detailed information of TA system TA117811

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 673402..674039
• Replicon: chromosome
• Accession: NZ_CP035456
• Organism: Paenibacillus mucilaginosus strain KN-18

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A916K9N6
• Locus tag: ERY13_RS03100
• Protein ID: WP_014649298.1
• Coordinates: 673689..674039 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ERY13_RS03095
• Protein ID: WP_041617724.1
• Coordinates: 673402..673683 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ERY13_RS03065 (ERY13_03020)	668868..669575	+	708	WP_014368380.1	glycosyltransferase family 2 protein	-
ERY13_RS03070 (ERY13_03025)	669578..669919	+	342	WP_041619021.1	DUF2304 domain-containing protein	-
ERY13_RS03075 (ERY13_03030)	669912..670247	+	336	WP_081484299.1	EamA family transporter	-
ERY13_RS03080 (ERY13_03035)	670379..671452	+	1074	WP_277713373.1	outer membrane lipoprotein carrier protein LolA	-
ERY13_RS03085	671449..671775	+	327	WP_277713374.1	DUF4367 domain-containing protein	-
ERY13_RS03090 (ERY13_03040)	671974..673158	+	1185	WP_014368382.1	alanine racemase	-
ERY13_RS03095 (ERY13_03045)	673402..673683	+	282	WP_041617724.1	CopG family ribbon-helix-helix protein	Antitoxin
ERY13_RS03100 (ERY13_03050)	673689..674039	+	351	WP_014649298.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ERY13_RS03105 (ERY13_03055)	674134..676350	+	2217	WP_014368384.1	Tex family protein	-
ERY13_RS03110 (ERY13_03060)	676520..676672	-	153	WP_143768091.1	cortex morphogenetic protein CmpA	-
ERY13_RS03115 (ERY13_03065)	676770..677138	-	369	WP_013914414.1	hypothetical protein	-
ERY13_RS03120 (ERY13_03070)	677294..677770	+	477	WP_014368385.1	SprT family protein	-
ERY13_RS03145 (ERY13_03095)	678601..679020	+	420	WP_013914416.1	pyridoxamine 5'-phosphate oxidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-46)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12890.84 Da
• Isoelectric Point: 4.8837

>T117811 WP_014649298.1 NZ_CP035456:673689-674039 [Paenibacillus mucilaginosus]
MIVKRGDVFFADLSPVVGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAEAHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEETMRKVDDALQISVGLIEF

Nucleotide

• Length: 351 bp

>T117811 NZ_CP035456:673689-674039 [Paenibacillus mucilaginosus]
TTGATTGTCAAACGTGGCGATGTCTTTTTCGCAGATCTGTCCCCCGTGGTGGGGTCCGAGCAGGGCGGTATCCGGCCCGT
TCTGATCATTCAGAATGATATCGGTAACAGATTCAGTCCCACGGTCATCGTGGCGGCCATCACGGCCCAGATTCAGAAAG
CCAAGCTGCCCACGCATGTGGAGATCGACGCGGAAGCGCACGGTTTTGACAGGGATTCCGTCATCCTTCTGGAGCAGATC
CGCACCATAGACAAACAGCGTCTTACAGATAAGATTACCCATCTGGATGAAGAAACCATGCGCAAGGTCGATGACGCCCT
TCAGATAAGTGTCGGACTGATTGAATTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10939.53 Da
• Isoelectric Point: 6.7286

>AT117811 WP_041617724.1 NZ_CP035456:673402-673683 [Paenibacillus mucilaginosus]
VANVQNTKRIMISLPDHLLQEVDYLVEKENSNRSEFIRQAMKLYLTERKKRHLRETMQRGYMEMAKINLHMASEAFQAEE
EADTTLDRLVSGV

Nucleotide

• Length: 282 bp

>AT117811 NZ_CP035456:673402-673683 [Paenibacillus mucilaginosus]
GTGGCCAATGTGCAGAACACGAAACGAATTATGATCAGTTTACCGGACCATTTGCTGCAGGAGGTGGACTATCTCGTGGA
AAAGGAAAATTCCAATCGGAGCGAGTTCATCCGACAAGCGATGAAGCTTTATTTGACTGAGCGCAAGAAGCGGCATTTGC
GTGAAACCATGCAGCGTGGATACATGGAAATGGCCAAGATCAACCTTCATATGGCCTCGGAAGCATTCCAAGCGGAGGAA
GAGGCGGATACCACGCTTGACCGTCTGGTGAGCGGGGTGTGA