Detailed information of TA system TA117793

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 272508..273616
• Replicon: chromosome
• Accession: NZ_CP035438
• Organism: Streptococcus pyogenes strain emm22.8

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: ETT61_RS01530
• Protein ID: WP_000233000.1
• Coordinates: 272747..273616 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: ETT61_RS01525
• Protein ID: WP_000205227.1
• Coordinates: 272508..272732 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ETT61_RS01490	267553..267939	+	387	WP_094983359.1	type II toxin-antitoxin system HicB family antitoxin	-
ETT61_RS10070	268097..268267	+	171	WP_000713595.1	hypothetical protein	-
ETT61_RS01495	268281..268883	+	603	WP_001062586.1	recombinase family protein	-
ETT61_RS01500	268899..269576	+	678	Protein_240	DNA topoisomerase	-
ETT61_RS01505	269887..270054	+	168	WP_000898188.1	hypothetical protein	-
ETT61_RS01510	270088..270384	+	297	WP_002360685.1	hypothetical protein	-
ETT61_RS01515	270385..270801	+	417	WP_000323438.1	recombinase	-
ETT61_RS01520	270803..272365	+	1563	WP_000136908.1	recombinase family protein	-
ETT61_RS01525	272508..272732	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
ETT61_RS01530	272747..273616	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
ETT61_RS01535	273597..274331	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
ETT61_RS01540	274364..275272	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
ETT61_RS01545	275269..275749	+	481	Protein_249	GNAT family N-acetyltransferase	-
ETT61_RS01550	275842..276636	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
ETT61_RS01555	277046..277131	+	86	Protein_251	peptide-binding protein	-
ETT61_RS01560	277180..277263	+	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
ETT61_RS01565	277388..278125	+	738	WP_010725416.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
ETT61_RS01575	278295..278555	+	261	Protein_254	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ant(6)-Ia / aph(3')-III / erm(B) / cat(pC194)	slo / speG / hasC	154659..327393	172734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T117793 WP_000233000.1 NZ_CP035438:272747-273616 [Streptococcus pyogenes]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T117793 NZ_CP035438:272747-273616 [Streptococcus pyogenes]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT117793 WP_000205227.1 NZ_CP035438:272508-272732 [Streptococcus pyogenes]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT117793 NZ_CP035438:272508-272732 [Streptococcus pyogenes]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: R3JIN1