Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 520545..521181 Replicon chromosome
Accession NZ_CP035413
Organism Bacillus subtilis strain SRCM103629

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ETL58_RS02710 Protein ID WP_003156187.1
Coordinates 520831..521181 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag ETL58_RS02705 Protein ID WP_003225183.1
Coordinates 520545..520826 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ETL58_RS02685 516904..517503 - 600 WP_129010114.1 rhomboid family intramembrane serine protease -
ETL58_RS02690 517598..517963 + 366 WP_014475874.1 holo-ACP synthase -
ETL58_RS02695 518129..519145 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
ETL58_RS02700 519260..520429 + 1170 WP_015252766.1 alanine racemase -
ETL58_RS02705 520545..520826 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ETL58_RS02710 520831..521181 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ETL58_RS02715 521296..522120 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
ETL58_RS02720 522125..522490 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
ETL58_RS02725 522494..522895 + 402 WP_129010115.1 serine/threonine-protein kinase RsbT -
ETL58_RS02730 522907..523914 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
ETL58_RS02735 523976..524305 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
ETL58_RS02740 524302..524784 + 483 WP_003234299.1 anti-sigma B factor RsbW -
ETL58_RS02745 524750..525538 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
ETL58_RS02750 525538..526137 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117772 WP_003156187.1 NZ_CP035413:520831-521181 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117772 NZ_CP035413:520831-521181 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT117772 WP_003225183.1 NZ_CP035413:520545-520826 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117772 NZ_CP035413:520545-520826 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References