Detailed information of TA system TA117765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1434226..1435143
• Replicon: chromosome
• Accession: NZ_CP035410
• Organism: Bacillus velezensis strain SRCM103616

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: ETK69_RS07680
• Protein ID: WP_007407256.1
• Coordinates: 1434397..1435143 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: ETK69_RS07675
• Protein ID: WP_003154807.1
• Coordinates: 1434226..1434396 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ETK69_RS07630	1429432..1429944	+	513	WP_017417605.1	sigma-70 family RNA polymerase sigma factor	-
ETK69_RS07635	1430057..1430716	+	660	WP_017417606.1	terminase	-
ETK69_RS07640	1430614..1431081	+	468	WP_076984018.1	hypothetical protein	-
ETK69_RS07645	1431094..1431465	+	372	WP_129093699.1	hypothetical protein	-
ETK69_RS07650	1431470..1431667	+	198	WP_007610833.1	XkdX family protein	-
ETK69_RS07655	1431724..1432485	+	762	WP_063636818.1	hypothetical protein	-
ETK69_RS07660	1432537..1432800	+	264	WP_021493821.1	hemolysin XhlA family protein	-
ETK69_RS07665	1432814..1433077	+	264	WP_003154813.1	phage holin	-
ETK69_RS07670	1433091..1433969	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
ETK69_RS07675	1434226..1434396	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
ETK69_RS07680	1434397..1435143	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
ETK69_RS07685	1435247..1436245	-	999	WP_017417610.1	inorganic phosphate transporter	-
ETK69_RS07690	1436258..1436875	-	618	WP_003154804.1	DUF47 domain-containing protein	-
ETK69_RS07695	1437162..1438478	-	1317	WP_063636817.1	amino acid permease	-
ETK69_RS07700	1438800..1439750	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T117765 WP_007407256.1 NZ_CP035410:c1435143-1434397 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T117765 NZ_CP035410:c1435143-1434397 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAAAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT117765 WP_003154807.1 NZ_CP035410:c1434396-1434226 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT117765 NZ_CP035410:c1434396-1434226 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14