Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 558240..558876 Replicon chromosome
Accession NZ_CP035405
Organism Bacillus licheniformis strain SRCM103608

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag ETK49_RS02775 Protein ID WP_003179128.1
Coordinates 558526..558876 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag ETK49_RS02770 Protein ID WP_006638778.1
Coordinates 558240..558521 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ETK49_RS02750 553355..554836 + 1482 WP_009330132.1 PH domain-containing protein -
ETK49_RS02755 554833..555432 - 600 WP_003179118.1 rhomboid family intramembrane serine protease -
ETK49_RS02760 555775..556734 + 960 WP_152847888.1 outer membrane lipoprotein carrier protein LolA -
ETK49_RS02765 556959..558128 + 1170 WP_025807384.1 alanine racemase -
ETK49_RS02770 558240..558521 + 282 WP_006638778.1 hypothetical protein Antitoxin
ETK49_RS02775 558526..558876 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ETK49_RS02780 558994..559821 + 828 WP_003179130.1 RsbT co-antagonist protein RsbRA -
ETK49_RS02785 559825..560190 + 366 WP_003179132.1 STAS domain-containing protein -
ETK49_RS02790 560193..560594 + 402 WP_003179135.1 anti-sigma regulatory factor -
ETK49_RS02795 560605..561612 + 1008 WP_003179137.1 PP2C family protein-serine/threonine phosphatase -
ETK49_RS02800 561671..561997 + 327 WP_003179140.1 anti-sigma factor antagonist -
ETK49_RS02805 561997..562482 + 486 WP_003179142.1 anti-sigma B factor RsbW -
ETK49_RS02810 562448..563239 + 792 WP_003179144.1 RNA polymerase sigma factor SigB -
ETK49_RS02815 563236..563835 + 600 WP_003179145.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T117762 WP_003179128.1 NZ_CP035405:558526-558876 [Bacillus licheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T117762 NZ_CP035405:558526-558876 [Bacillus licheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT117762 WP_006638778.1 NZ_CP035405:558240-558521 [Bacillus licheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT117762 NZ_CP035405:558240-558521 [Bacillus licheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTACGCGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATCAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References