Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1427158..1428074 Replicon chromosome
Accession NZ_CP035403
Organism Bacillus subtilis strain SRCM103581

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag ETA15_RS07630 Protein ID WP_003244695.1
Coordinates 1427328..1428074 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag ETA15_RS07625 Protein ID WP_003232646.1
Coordinates 1427158..1427328 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ETA15_RS07590 1424021..1424350 + 330 WP_003232660.1 phage-like element PBSX protein XkdW -
ETA15_RS07595 1424347..1424511 + 165 WP_087960875.1 XkdX family protein -
ETA15_RS07600 1424555..1425393 + 839 Protein_1424 phage-like element PBSX protein XepA -
ETA15_RS07605 1425446..1425715 + 270 WP_003232655.1 hemolysin XhlA family protein -
ETA15_RS07610 1425728..1425991 + 264 WP_003232653.1 phage holin -
ETA15_RS07615 1426004..1426897 + 894 WP_014479567.1 N-acetylmuramoyl-L-alanine amidase -
ETA15_RS07620 1426935..1427072 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
ETA15_RS07625 1427158..1427328 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
ETA15_RS07630 1427328..1428074 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
ETA15_RS07635 1428184..1429185 - 1002 WP_014479569.1 inorganic phosphate transporter -
ETA15_RS07640 1429198..1429815 - 618 WP_003218470.1 DUF47 domain-containing protein -
ETA15_RS07645 1430091..1431407 - 1317 WP_041333863.1 serine/threonine exchanger -
ETA15_RS07650 1431796..1432746 + 951 WP_014476561.1 ring-cleaving dioxygenase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-238)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T117759 WP_003244695.1 NZ_CP035403:c1428074-1427328 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T117759 NZ_CP035403:c1428074-1427328 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAACTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCCTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCCTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT117759 WP_003232646.1 NZ_CP035403:c1427328-1427158 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT117759 NZ_CP035403:c1427328-1427158 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 3O6Q


Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References