Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 558722..559358 Replicon chromosome
Accession NZ_CP035403
Organism Bacillus subtilis strain SRCM103581

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ETA15_RS02815 Protein ID WP_003156187.1
Coordinates 559008..559358 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag ETA15_RS02810 Protein ID WP_003225183.1
Coordinates 558722..559003 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ETA15_RS02790 555082..555681 - 600 WP_017697004.1 rhomboid family intramembrane serine protease -
ETA15_RS02795 555776..556141 + 366 WP_014475874.1 holo-ACP synthase -
ETA15_RS02800 556307..557323 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
ETA15_RS02805 557437..558606 + 1170 WP_017697002.1 alanine racemase -
ETA15_RS02810 558722..559003 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ETA15_RS02815 559008..559358 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ETA15_RS02820 559473..560297 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
ETA15_RS02825 560302..560667 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
ETA15_RS02830 560671..561072 + 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
ETA15_RS02835 561084..562091 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
ETA15_RS02840 562153..562482 + 330 WP_014662855.1 anti-sigma factor antagonist RsbV -
ETA15_RS02845 562479..562961 + 483 WP_003234299.1 anti-sigma B factor RsbW -
ETA15_RS02850 562927..563715 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
ETA15_RS02855 563715..564314 + 600 WP_017696999.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117757 WP_003156187.1 NZ_CP035403:559008-559358 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117757 NZ_CP035403:559008-559358 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT117757 WP_003225183.1 NZ_CP035403:558722-559003 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117757 NZ_CP035403:558722-559003 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References