Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 510336..510972 Replicon chromosome
Accession NZ_CP035402
Organism Bacillus subtilis strain SRCM103576

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ES969_RS02650 Protein ID WP_003156187.1
Coordinates 510622..510972 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag ES969_RS02645 Protein ID WP_003225183.1
Coordinates 510336..510617 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ES969_RS02625 506695..507294 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
ES969_RS02630 507389..507754 + 366 WP_014478897.1 holo-ACP synthase -
ES969_RS02635 507920..508936 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
ES969_RS02640 509051..510220 + 1170 WP_014478898.1 alanine racemase -
ES969_RS02645 510336..510617 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ES969_RS02650 510622..510972 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ES969_RS02655 511077..511901 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
ES969_RS02660 511906..512271 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
ES969_RS02665 512275..512676 + 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
ES969_RS02670 512688..513695 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
ES969_RS02675 513757..514086 + 330 WP_014662855.1 anti-sigma factor antagonist RsbV -
ES969_RS02680 514083..514565 + 483 WP_003234299.1 anti-sigma B factor RsbW -
ES969_RS02685 514531..515319 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
ES969_RS02690 515319..515918 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117755 WP_003156187.1 NZ_CP035402:510622-510972 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117755 NZ_CP035402:510622-510972 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT117755 WP_003225183.1 NZ_CP035402:510336-510617 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117755 NZ_CP035402:510336-510617 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References