Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 521227..521863 Replicon chromosome
Accession NZ_CP035400
Organism Bacillus subtilis strain SRCM103835

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ETA18_RS02680 Protein ID WP_003156187.1
Coordinates 521513..521863 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag ETA18_RS02675 Protein ID WP_003225183.1
Coordinates 521227..521508 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ETA18_RS02655 517586..518185 - 600 WP_128992430.1 rhomboid family intramembrane serine protease -
ETA18_RS02660 518280..518645 + 366 WP_014475874.1 holo-ACP synthase -
ETA18_RS02665 518811..519827 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
ETA18_RS02670 519942..521111 + 1170 WP_106074084.1 alanine racemase -
ETA18_RS02675 521227..521508 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ETA18_RS02680 521513..521863 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ETA18_RS02685 521978..522802 + 825 WP_128992431.1 RsbT co-antagonist protein RsbRA -
ETA18_RS02690 522807..523172 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
ETA18_RS02695 523176..523577 + 402 WP_017697001.1 serine/threonine-protein kinase RsbT -
ETA18_RS02700 523589..524596 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
ETA18_RS02705 524658..524987 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
ETA18_RS02710 524984..525466 + 483 WP_003234299.1 anti-sigma B factor RsbW -
ETA18_RS02715 525432..526220 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
ETA18_RS02720 526220..526819 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117751 WP_003156187.1 NZ_CP035400:521513-521863 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117751 NZ_CP035400:521513-521863 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT117751 WP_003225183.1 NZ_CP035400:521227-521508 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117751 NZ_CP035400:521227-521508 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References