Detailed information of TA system TA117750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1380404..1381321
• Replicon: chromosome
• Accession: NZ_CP035399
• Organism: Bacillus velezensis strain SRCM103788

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: ETA12_RS07310
• Protein ID: WP_007407256.1
• Coordinates: 1380575..1381321 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: ETA12_RS07305
• Protein ID: WP_003154807.1
• Coordinates: 1380404..1380574 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ETA12_RS07260	1375610..1376122	+	513	WP_017417605.1	sigma-70 family RNA polymerase sigma factor	-
ETA12_RS07265	1376235..1376894	+	660	WP_017417606.1	terminase	-
ETA12_RS07270	1376792..1377259	+	468	WP_076984018.1	hypothetical protein	-
ETA12_RS07275	1377272..1377643	+	372	WP_129093699.1	hypothetical protein	-
ETA12_RS07280	1377648..1377845	+	198	WP_007610833.1	XkdX family protein	-
ETA12_RS07285	1377902..1378663	+	762	WP_063636818.1	hypothetical protein	-
ETA12_RS07290	1378715..1378978	+	264	WP_021493821.1	hemolysin XhlA family protein	-
ETA12_RS07295	1378992..1379255	+	264	WP_003154813.1	phage holin	-
ETA12_RS07300	1379269..1380147	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
ETA12_RS07305	1380404..1380574	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
ETA12_RS07310	1380575..1381321	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
ETA12_RS07315	1381425..1382423	-	999	WP_017417610.1	inorganic phosphate transporter	-
ETA12_RS07320	1382436..1383053	-	618	WP_003154804.1	DUF47 domain-containing protein	-
ETA12_RS07325	1383340..1384656	-	1317	WP_063636817.1	amino acid permease	-
ETA12_RS07330	1384978..1385928	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T117750 WP_007407256.1 NZ_CP035399:c1381321-1380575 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T117750 NZ_CP035399:c1381321-1380575 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAAAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT117750 WP_003154807.1 NZ_CP035399:c1380574-1380404 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT117750 NZ_CP035399:c1380574-1380404 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14