Detailed information of TA system TA117749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 493925..494562
• Replicon: chromosome
• Accession: NZ_CP035399
• Organism: Bacillus velezensis strain SRCM103788

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: ETA12_RS02510
• Protein ID: WP_003156187.1
• Coordinates: 494212..494562 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: ETA12_RS02505
• Protein ID: WP_003156188.1
• Coordinates: 493925..494206 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ETA12_RS02485	490290..490889	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
ETA12_RS02490	490982..491347	+	366	WP_007609580.1	holo-ACP synthase	-
ETA12_RS02495	491512..492519	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
ETA12_RS02500	492636..493805	+	1170	WP_025649966.1	alanine racemase	-
ETA12_RS02505	493925..494206	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
ETA12_RS02510	494212..494562	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
ETA12_RS02515	494680..495501	+	822	WP_025649964.1	STAS domain-containing protein	-
ETA12_RS02520	495506..495871	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
ETA12_RS02525	495874..496275	+	402	WP_003156178.1	anti-sigma regulatory factor	-
ETA12_RS02530	496287..497294	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
ETA12_RS02535	497358..497687	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
ETA12_RS02540	497684..498166	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
ETA12_RS02545	498132..498920	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
ETA12_RS02550	498920..499522	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T117749 WP_003156187.1 NZ_CP035399:494212-494562 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T117749 NZ_CP035399:494212-494562 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT117749 WP_003156188.1 NZ_CP035399:493925-494206 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT117749 NZ_CP035399:493925-494206 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5