Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1341914..1342830 Replicon chromosome
Accession NZ_CP035397
Organism Bacillus subtilis strain SRCM103773

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag ETA10_RS07115 Protein ID WP_003244695.1
Coordinates 1342084..1342830 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag ETA10_RS07110 Protein ID WP_003232646.1
Coordinates 1341914..1342084 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ETA10_RS07075 1338774..1339103 + 330 WP_046380959.1 XkdW family protein -
ETA10_RS07080 1339100..1339264 + 165 WP_014479563.1 XkdX family protein -
ETA10_RS07085 1339311..1340150 + 840 WP_046380960.1 phage-like element PBSX protein XepA -
ETA10_RS07090 1340203..1340472 + 270 WP_015252265.1 hemolysin XhlA family protein -
ETA10_RS07095 1340485..1340748 + 264 WP_003232653.1 phage holin -
ETA10_RS07100 1340761..1341654 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
ETA10_RS07105 1341691..1341828 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
ETA10_RS07110 1341914..1342084 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
ETA10_RS07115 1342084..1342830 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
ETA10_RS07120 1342940..1343941 - 1002 WP_003232642.1 inorganic phosphate transporter -
ETA10_RS07125 1343954..1344571 - 618 WP_003218470.1 DUF47 domain-containing protein -
ETA10_RS07130 1344847..1346163 - 1317 WP_046380961.1 serine/threonine exchanger -
ETA10_RS07135 1346552..1347502 + 951 WP_014479571.1 ring-cleaving dioxygenase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1306878..1363275 56397


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-238)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T117744 WP_003244695.1 NZ_CP035397:c1342830-1342084 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T117744 NZ_CP035397:c1342830-1342084 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTATATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT117744 WP_003232646.1 NZ_CP035397:c1342084-1341914 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT117744 NZ_CP035397:c1342084-1341914 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 3O6Q


Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References