Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 519281..519917 Replicon chromosome
Accession NZ_CP035397
Organism Bacillus subtilis strain SRCM103773

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag ETA10_RS02685 Protein ID WP_003156187.1
Coordinates 519567..519917 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag ETA10_RS02680 Protein ID WP_003225183.1
Coordinates 519281..519562 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ETA10_RS02660 515640..516239 - 600 WP_032722928.1 rhomboid family intramembrane serine protease -
ETA10_RS02665 516334..516699 + 366 WP_015252768.1 holo-ACP synthase -
ETA10_RS02670 516865..517881 + 1017 WP_080019556.1 outer membrane lipoprotein carrier protein LolA -
ETA10_RS02675 517996..519165 + 1170 WP_015252766.1 alanine racemase -
ETA10_RS02680 519281..519562 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
ETA10_RS02685 519567..519917 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
ETA10_RS02690 520032..520856 + 825 WP_015252765.1 RsbT co-antagonist protein RsbRA -
ETA10_RS02695 520861..521226 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
ETA10_RS02700 521230..521631 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
ETA10_RS02705 521643..522650 + 1008 WP_015252763.1 phosphoserine phosphatase RsbU -
ETA10_RS02710 522712..523041 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
ETA10_RS02715 523038..523520 + 483 WP_003234299.1 anti-sigma B factor RsbW -
ETA10_RS02720 523486..524274 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
ETA10_RS02725 524274..524873 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T117743 WP_003156187.1 NZ_CP035397:519567-519917 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T117743 NZ_CP035397:519567-519917 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT117743 WP_003225183.1 NZ_CP035397:519281-519562 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT117743 NZ_CP035397:519281-519562 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References